Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion

Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion

Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardwa...

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Main Authors: Sue, Mian Then, Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J., Stodieck, Louis, Rahman Jamal
Format: Article
Language:English
Published: Pusat Perubatan Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/13238/
http://journalarticle.ukm.my/13238/
http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf
id ukm-13238
recordtype eprints
spelling ukm-132382019-07-29T04:38:36Z http://journalarticle.ukm.my/13238/ Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion Sue, Mian Then Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J. Stodieck, Louis Rahman Jamal, Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardware. The CHab was flown on the STS116 flight to the International Space Station (ISS) and returned to earth on the STS118 flight. Upon landing, surviving worms were extracted and RN Alater added. Ground controls were grown at Bioserve in Colorado and passaged synchronously. RN A was later extracted and mRN A gene expression was analysed using Affymetrix GeneChip® C. elegans Genome Array. The results revealed that 858 known genes were differentially expressed (p-value ≤ 0.05 and fold change ≥ ± 2); namely 608 genes were up-regulated and 250 genes down-regulated. The genes dod-19 and dod-3 which are the downstream effectors of the forkhead transcription factor daf-16 were up-regulated. Daf-16 regulates insulin/TGF signaling pathway that influence metabolic alterations, increased stress and microbial resistance. The glutathione S-transferase (gst-1), Flavin-containing MonoOxygenase (fmo-3) and radiation sensitive genes (rad-51 and him-6) were all up-regulated suggesting responses to oxidative stress. The down-regulation of muscle-related genes (mua-3, col-97, col-109, and col-113) maybe due to reduced mechanical stress in muscle exposed to long-term microgravity. This was the longest exposure of a multi-generational cohort of C. elegans to microgravity which were passaged through at least 10 generations in space. The results suggest key changes in genes involved in ageing, DN A repair, oxidative stress and muscle growth. Pusat Perubatan Universiti Kebangsaan Malaysia 2014 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf Sue, Mian Then and Nurul-Faizah Jusoh, and Roslan Harun, and Sheila Nathan, and Szewczyk, Nathaniel J. and Stodieck, Louis and Rahman Jamal, (2014) Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion. Asia-Pacific Journal of Molecular Medicine, 4 (2). pp. 1-11. ISSN 2232-0326 http://spaj.ukm.my/apjmm/index.php/apjmm/issue/view/9
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardware. The CHab was flown on the STS116 flight to the International Space Station (ISS) and returned to earth on the STS118 flight. Upon landing, surviving worms were extracted and RN Alater added. Ground controls were grown at Bioserve in Colorado and passaged synchronously. RN A was later extracted and mRN A gene expression was analysed using Affymetrix GeneChip® C. elegans Genome Array. The results revealed that 858 known genes were differentially expressed (p-value ≤ 0.05 and fold change ≥ ± 2); namely 608 genes were up-regulated and 250 genes down-regulated. The genes dod-19 and dod-3 which are the downstream effectors of the forkhead transcription factor daf-16 were up-regulated. Daf-16 regulates insulin/TGF signaling pathway that influence metabolic alterations, increased stress and microbial resistance. The glutathione S-transferase (gst-1), Flavin-containing MonoOxygenase (fmo-3) and radiation sensitive genes (rad-51 and him-6) were all up-regulated suggesting responses to oxidative stress. The down-regulation of muscle-related genes (mua-3, col-97, col-109, and col-113) maybe due to reduced mechanical stress in muscle exposed to long-term microgravity. This was the longest exposure of a multi-generational cohort of C. elegans to microgravity which were passaged through at least 10 generations in space. The results suggest key changes in genes involved in ageing, DN A repair, oxidative stress and muscle growth.
format Article
author Sue, Mian Then
Nurul-Faizah Jusoh,
Roslan Harun,
Sheila Nathan,
Szewczyk, Nathaniel J.
Stodieck, Louis
Rahman Jamal,
spellingShingle Sue, Mian Then
Nurul-Faizah Jusoh,
Roslan Harun,
Sheila Nathan,
Szewczyk, Nathaniel J.
Stodieck, Louis
Rahman Jamal,
Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
author_facet Sue, Mian Then
Nurul-Faizah Jusoh,
Roslan Harun,
Sheila Nathan,
Szewczyk, Nathaniel J.
Stodieck, Louis
Rahman Jamal,
author_sort Sue, Mian Then
title Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
title_short Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
title_full Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
title_fullStr Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
title_full_unstemmed Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
title_sort multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, dna repair,and locomotion
publisher Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/13238/
http://journalarticle.ukm.my/13238/
http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf
first_indexed 2023-09-18T20:04:24Z
last_indexed 2023-09-18T20:04:24Z
_version_ 1777407062109585408

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