Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing

Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned M...

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Main Authors:	Shiplu Roy Chowdhury, Lim, Shaun Jing, Muhammad Noor Haziq Zolkafli, Nur’ Aqilah Mohd Abu Zarin, Wan Alyani Wan Abdullah, Nurul Aidah Md Mothar, Manira Maarof, Nur Atiqah Haizum Abdullah
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13323/ http://journalarticle.ukm.my/13323/ http://journalarticle.ukm.my/13323/1/17%20Shiplu%20Roy%20Chowdhury.pdf

id	ukm-13323
recordtype	eprints
spelling	ukm-133232019-08-29T21:40:16Z http://journalarticle.ukm.my/13323/ Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing Shiplu Roy Chowdhury, Lim, Shaun Jing Muhammad Noor Haziq Zolkafli, Nur’ Aqilah Mohd Abu Zarin, Wan Alyani Wan Abdullah, Nurul Aidah Md Mothar, Manira Maarof, Nur Atiqah Haizum Abdullah, Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned Medium (DFCM) and evaluate its potential on in vitro skin wound healing and anti-ageing properties. DFCM were obtained by culturing confluent human dermal fibroblasts (n=3) in keratinocyte-specific medium (DFCM-KM), fibroblast-specific medium (DFCM-FM) and fibroblast-specific medium with growth supplements (DFCM-GM). Protein concentration was determined by Bicinchoninic Acid (BCA) assay. To evaluate the wound healing and anti-ageing properties, human dermal fibroblasts were supplemented with three different DFCM. Fibroblasts cultured with F12:DMEM+10%FBS was used as a control. The growth rate of fibroblasts was evaluated by culturing cells until day 4. Migration rate was evaluated at 12 h after seeding. In vitro healing rate was evaluated via scratch assay. Anti-ageing potential of DFCM was evaluated via RT2 Profiler PCR Array. Concentration of total protein was found to be significantly higher in DFCM-GM (2914.79±150.67mg/mL) compared to DFCM-KM and DFCM-FM. Out of the tested 12 anti-ageing genes, fibroblasts supplemented with DFCM-GM demonstrated a significantly higher expression of WRN compared to other conditions. In conclusion, DFCM-GM contains more proteins and found superior in modulating wound healing and anti-ageing properties. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13323/1/17%20Shiplu%20Roy%20Chowdhury.pdf Shiplu Roy Chowdhury, and Lim, Shaun Jing and Muhammad Noor Haziq Zolkafli, and Nur’ Aqilah Mohd Abu Zarin, and Wan Alyani Wan Abdullah, and Nurul Aidah Md Mothar, and Manira Maarof, and Nur Atiqah Haizum Abdullah, (2019) Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing. Sains Malaysiana, 48 (3). pp. 637-644. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned Medium (DFCM) and evaluate its potential on in vitro skin wound healing and anti-ageing properties. DFCM were obtained by culturing confluent human dermal fibroblasts (n=3) in keratinocyte-specific medium (DFCM-KM), fibroblast-specific medium (DFCM-FM) and fibroblast-specific medium with growth supplements (DFCM-GM). Protein concentration was determined by Bicinchoninic Acid (BCA) assay. To evaluate the wound healing and anti-ageing properties, human dermal fibroblasts were supplemented with three different DFCM. Fibroblasts cultured with F12:DMEM+10%FBS was used as a control. The growth rate of fibroblasts was evaluated by culturing cells until day 4. Migration rate was evaluated at 12 h after seeding. In vitro healing rate was evaluated via scratch assay. Anti-ageing potential of DFCM was evaluated via RT2 Profiler PCR Array. Concentration of total protein was found to be significantly higher in DFCM-GM (2914.79±150.67mg/mL) compared to DFCM-KM and DFCM-FM. Out of the tested 12 anti-ageing genes, fibroblasts supplemented with DFCM-GM demonstrated a significantly higher expression of WRN compared to other conditions. In conclusion, DFCM-GM contains more proteins and found superior in modulating wound healing and anti-ageing properties.
format	Article
author	Shiplu Roy Chowdhury, Lim, Shaun Jing Muhammad Noor Haziq Zolkafli, Nur’ Aqilah Mohd Abu Zarin, Wan Alyani Wan Abdullah, Nurul Aidah Md Mothar, Manira Maarof, Nur Atiqah Haizum Abdullah,
spellingShingle	Shiplu Roy Chowdhury, Lim, Shaun Jing Muhammad Noor Haziq Zolkafli, Nur’ Aqilah Mohd Abu Zarin, Wan Alyani Wan Abdullah, Nurul Aidah Md Mothar, Manira Maarof, Nur Atiqah Haizum Abdullah, Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
author_facet	Shiplu Roy Chowdhury, Lim, Shaun Jing Muhammad Noor Haziq Zolkafli, Nur’ Aqilah Mohd Abu Zarin, Wan Alyani Wan Abdullah, Nurul Aidah Md Mothar, Manira Maarof, Nur Atiqah Haizum Abdullah,
author_sort	Shiplu Roy Chowdhury,
title	Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
title_short	Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
title_full	Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
title_fullStr	Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
title_full_unstemmed	Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
title_sort	exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13323/ http://journalarticle.ukm.my/13323/ http://journalarticle.ukm.my/13323/1/17%20Shiplu%20Roy%20Chowdhury.pdf
first_indexed	2023-09-18T20:04:36Z
last_indexed	2023-09-18T20:04:36Z
_version_	1777407073888239616

Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing

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