Development of sperm separation system using electrical current for bull

A novel electrophoretic separation system has been successfully applied for the preparation of human sperm prior to the execution of assisted reproductive techniques (ARTs). This new system is designed to overcome the generation of reactive oxygen species (ROS) through centrifugation in conventional...

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Main Authors:	Farah Hanan Fathihah Jaffar, Siti Fatimah Ibrahim, Mohd Iswadi Ismail, Chew, Fang Nang, Syarifah Faezah Syed Mohamad, Nur Hilwani Ismail, Nurol Afiza Abdul Wahab, Balkhis Bashuri, Khairul Osman
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13344/ http://journalarticle.ukm.my/13344/ http://journalarticle.ukm.my/13344/1/17389-92208-1-PB.pdf

id	ukm-13344
recordtype	eprints
spelling	ukm-133442019-09-06T22:19:08Z http://journalarticle.ukm.my/13344/ Development of sperm separation system using electrical current for bull Farah Hanan Fathihah Jaffar, Siti Fatimah Ibrahim, Mohd Iswadi Ismail, Chew, Fang Nang Syarifah Faezah Syed Mohamad, Nur Hilwani Ismail, Nurol Afiza Abdul Wahab, Balkhis Bashuri, Khairul Osman, A novel electrophoretic separation system has been successfully applied for the preparation of human sperm prior to the execution of assisted reproductive techniques (ARTs). This new system is designed to overcome the generation of reactive oxygen species (ROS) through centrifugation in conventional sperm preparation. Since the previous study showed favorable outcomes in humans, this study intends to implement this new system for animal sperm preparation particularly in bull. Fresh semen from adult bulls were used. Optimization of the electrophoretic system for optimum bull sperm separation involved different strength of voltage and separation time. The voltages applied were 10V, 20V, 30V, 40V, 50V, and 60V. For each voltage applied, the system was operated for a duration of 12 min. An average of 10 μl fractionalized semen was taken out at the collection site at every 2-min interval. Every fractionated sperm was then evaluated for percentage of viability, motility, and DNA damage assessment. Result showed that electrophoresis at 20V and 6 min yielded more than 80% viable and more than 70% motile sperm population with the lowest DNA damage. In conclusion, the system was able to fractionate high quality bull sperm at 20V and 6 min. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13344/1/17389-92208-1-PB.pdf Farah Hanan Fathihah Jaffar, and Siti Fatimah Ibrahim, and Mohd Iswadi Ismail, and Chew, Fang Nang and Syarifah Faezah Syed Mohamad, and Nur Hilwani Ismail, and Nurol Afiza Abdul Wahab, and Balkhis Bashuri, and Khairul Osman, (2019) Development of sperm separation system using electrical current for bull. Jurnal Sains Kesihatan Malaysia, 17 (1). pp. 99-105. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/686
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A novel electrophoretic separation system has been successfully applied for the preparation of human sperm prior to the execution of assisted reproductive techniques (ARTs). This new system is designed to overcome the generation of reactive oxygen species (ROS) through centrifugation in conventional sperm preparation. Since the previous study showed favorable outcomes in humans, this study intends to implement this new system for animal sperm preparation particularly in bull. Fresh semen from adult bulls were used. Optimization of the electrophoretic system for optimum bull sperm separation involved different strength of voltage and separation time. The voltages applied were 10V, 20V, 30V, 40V, 50V, and 60V. For each voltage applied, the system was operated for a duration of 12 min. An average of 10 μl fractionalized semen was taken out at the collection site at every 2-min interval. Every fractionated sperm was then evaluated for percentage of viability, motility, and DNA damage assessment. Result showed that electrophoresis at 20V and 6 min yielded more than 80% viable and more than 70% motile sperm population with the lowest DNA damage. In conclusion, the system was able to fractionate high quality bull sperm at 20V and 6 min.
format	Article
author	Farah Hanan Fathihah Jaffar, Siti Fatimah Ibrahim, Mohd Iswadi Ismail, Chew, Fang Nang Syarifah Faezah Syed Mohamad, Nur Hilwani Ismail, Nurol Afiza Abdul Wahab, Balkhis Bashuri, Khairul Osman,
spellingShingle	Farah Hanan Fathihah Jaffar, Siti Fatimah Ibrahim, Mohd Iswadi Ismail, Chew, Fang Nang Syarifah Faezah Syed Mohamad, Nur Hilwani Ismail, Nurol Afiza Abdul Wahab, Balkhis Bashuri, Khairul Osman, Development of sperm separation system using electrical current for bull
author_facet	Farah Hanan Fathihah Jaffar, Siti Fatimah Ibrahim, Mohd Iswadi Ismail, Chew, Fang Nang Syarifah Faezah Syed Mohamad, Nur Hilwani Ismail, Nurol Afiza Abdul Wahab, Balkhis Bashuri, Khairul Osman,
author_sort	Farah Hanan Fathihah Jaffar,
title	Development of sperm separation system using electrical current for bull
title_short	Development of sperm separation system using electrical current for bull
title_full	Development of sperm separation system using electrical current for bull
title_fullStr	Development of sperm separation system using electrical current for bull
title_full_unstemmed	Development of sperm separation system using electrical current for bull
title_sort	development of sperm separation system using electrical current for bull
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13344/ http://journalarticle.ukm.my/13344/ http://journalarticle.ukm.my/13344/1/17389-92208-1-PB.pdf
first_indexed	2023-09-18T20:04:38Z
last_indexed	2023-09-18T20:04:38Z
_version_	1777407076832641024

Development of sperm separation system using electrical current for bull

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