Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin

Enterobacter aerogenes (Strain EA1) was characterised as gelatinase species-specific bacterium to porcine and fish gelatin. This bacterium offers the possibility of enzymes production to each species gelatin. In this study, a bioinformatic analysis toward genome sequence of E. aerogenes was perfomed...

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Main Authors:	Safiyyah Shahimi, Sahilah Abd. Mutalib, Rozida Mohd. Khalid, Mohd Fadly Lamri, Maaruf Abdul Ghani, Mohd. Faizal Abu Bakar, Mohd. Noor Mat Isa
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13380/ http://journalarticle.ukm.my/13380/ http://journalarticle.ukm.my/13380/1/09%20Safiyyah%20Shahimi.pdf

id	ukm-13380
recordtype	eprints
spelling	ukm-133802019-09-19T22:09:39Z http://journalarticle.ukm.my/13380/ Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin Safiyyah Shahimi, Sahilah Abd. Mutalib, Rozida Mohd. Khalid, Mohd Fadly Lamri, Maaruf Abdul Ghani, Mohd. Faizal Abu Bakar, Mohd. Noor Mat Isa, Enterobacter aerogenes (Strain EA1) was characterised as gelatinase species-specific bacterium to porcine and fish gelatin. This bacterium offers the possibility of enzymes production to each species gelatin. In this study, a bioinformatic analysis toward genome sequence of E. aerogenes was perfomed to determine genes encoded for gelatinase. E. aerogenes were partially genome sequenced resulting in 5.0 mega basepair total size of sequence. From pre-process pipeline, 78.58% of high quality reads was obtained for E. aerogenes. Genome assembly produced 120 contigs with 55.17% of GC base content. Protein prediction analysis determined 4705 genes from E. aerogenes. Two gelatinase candidate target genes selected from E. aerogenes were NODE_9_length_26866_cov_148.013245_12 containing 1029 bp sequence and NODE_24_length_155103_cov_177.082458_62 containing 717 bp sequence with highest similarity identity percentage against gelatinase enzyme available in Swiss-Prot and NCBI online database. Then, primers were designed for each target genes based on the open reading frame of selected genes. The expected gene candidate showed, 1029 bp and 717 bp amplicons for gen NODE_9_12 and gene NODE_24_62, respectively. Thus, gelatinase candidate encoded genes of E. aerogenes were identified for hydrolyzing porcine gelatin. Penerbit Universiti Kebangsaan Malaysia 2019-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13380/1/09%20Safiyyah%20Shahimi.pdf Safiyyah Shahimi, and Sahilah Abd. Mutalib, and Rozida Mohd. Khalid, and Mohd Fadly Lamri, and Maaruf Abdul Ghani, and Mohd. Faizal Abu Bakar, and Mohd. Noor Mat Isa, (2019) Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin. Sains Malaysiana, 48 (4). pp. 773-780. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil4_2019/KandunganJilid48Bil4_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Enterobacter aerogenes (Strain EA1) was characterised as gelatinase species-specific bacterium to porcine and fish gelatin. This bacterium offers the possibility of enzymes production to each species gelatin. In this study, a bioinformatic analysis toward genome sequence of E. aerogenes was perfomed to determine genes encoded for gelatinase. E. aerogenes were partially genome sequenced resulting in 5.0 mega basepair total size of sequence. From pre-process pipeline, 78.58% of high quality reads was obtained for E. aerogenes. Genome assembly produced 120 contigs with 55.17% of GC base content. Protein prediction analysis determined 4705 genes from E. aerogenes. Two gelatinase candidate target genes selected from E. aerogenes were NODE_9_length_26866_cov_148.013245_12 containing 1029 bp sequence and NODE_24_length_155103_cov_177.082458_62 containing 717 bp sequence with highest similarity identity percentage against gelatinase enzyme available in Swiss-Prot and NCBI online database. Then, primers were designed for each target genes based on the open reading frame of selected genes. The expected gene candidate showed, 1029 bp and 717 bp amplicons for gen NODE_9_12 and gene NODE_24_62, respectively. Thus, gelatinase candidate encoded genes of E. aerogenes were identified for hydrolyzing porcine gelatin.
format	Article
author	Safiyyah Shahimi, Sahilah Abd. Mutalib, Rozida Mohd. Khalid, Mohd Fadly Lamri, Maaruf Abdul Ghani, Mohd. Faizal Abu Bakar, Mohd. Noor Mat Isa,
spellingShingle	Safiyyah Shahimi, Sahilah Abd. Mutalib, Rozida Mohd. Khalid, Mohd Fadly Lamri, Maaruf Abdul Ghani, Mohd. Faizal Abu Bakar, Mohd. Noor Mat Isa, Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
author_facet	Safiyyah Shahimi, Sahilah Abd. Mutalib, Rozida Mohd. Khalid, Mohd Fadly Lamri, Maaruf Abdul Ghani, Mohd. Faizal Abu Bakar, Mohd. Noor Mat Isa,
author_sort	Safiyyah Shahimi,
title	Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
title_short	Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
title_full	Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
title_fullStr	Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
title_full_unstemmed	Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin
title_sort	representative candidate of gelatinase encoded gene in enterobacter aerogenes (strain ea1) for hydrolyzing porcine gelatin
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13380/ http://journalarticle.ukm.my/13380/ http://journalarticle.ukm.my/13380/1/09%20Safiyyah%20Shahimi.pdf
first_indexed	2023-09-18T20:04:44Z
last_indexed	2023-09-18T20:04:44Z
_version_	1777407082119561216

Representative candidate of gelatinase encoded gene in Enterobacter aerogenes (Strain EA1) for hydrolyzing porcine gelatin

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