Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)

Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in bri...

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Main Authors:	Mozhiarhasi Sandra Sagrin, Nur Fatimah Lasano, Radhiah Shukri, Nurul Shazini Ramli
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13386/ http://journalarticle.ukm.my/13386/ http://journalarticle.ukm.my/13386/1/15%20Mozhiarhasi%20Sandra%20Sagrin.pdf

id	ukm-13386
recordtype	eprints
spelling	ukm-133862019-09-20T23:08:54Z http://journalarticle.ukm.my/13386/ Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina) Mozhiarhasi Sandra Sagrin, Nur Fatimah Lasano, Radhiah Shukri, Nurul Shazini Ramli, Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in brine shrimp. The results showed that 100% ethanol leaf extract had the highest antioxidant activity with an IC50 value of 261.97 ± 0.57 μg/mL according to the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. Estimation of the total phenolic content (TPC) using the Folin-Ciocalteu reagent showed that the leaves extracted with 100% ethanol had the highest concentration of TPC compared to the extracts prepared with other solvents of all parts of the plant. Analysis of the minerals using inductively coupled plasma atomic emission spectroscopy (ICP-OES) showed that the parts of the plant primarily consist of high levels of phosphorus, magnesium and calcium. Titration analysis of vitamin C showed that the highest concentration of the vitamin is present in the bark. High performance liquid chromatography (HPLC) analysis of vitamin E indicated that the content is the highest in the fruit, and the content of vitamin A was the highest in the leaves. Brine shrimp lethality assay (BSLA) results showed that 50% ethanol extract of the leaves is the most toxic during a 24 h treatment. Thus, the leaves and bark exhibit excellent antioxidant effects and can be potentially developed as functional food ingredients. The findings of the present study suggest further research in cell lines and in vivo. Penerbit Universiti Kebangsaan Malaysia 2019-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13386/1/15%20Mozhiarhasi%20Sandra%20Sagrin.pdf Mozhiarhasi Sandra Sagrin, and Nur Fatimah Lasano, and Radhiah Shukri, and Nurul Shazini Ramli, (2019) Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina). Sains Malaysiana, 48 (4). pp. 831-840. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil4_2019/KandunganJilid48Bil4_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Crescentia cujete has traditionally been used to treat various ailments. The present study attempted to determine the antioxidant activities of 100% ethanol, 50% ethanol and aqueous extracts of the leaves, bark and fruit of the herb. Additionally, the toxicity of the extracts was investigated in brine shrimp. The results showed that 100% ethanol leaf extract had the highest antioxidant activity with an IC50 value of 261.97 ± 0.57 μg/mL according to the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. Estimation of the total phenolic content (TPC) using the Folin-Ciocalteu reagent showed that the leaves extracted with 100% ethanol had the highest concentration of TPC compared to the extracts prepared with other solvents of all parts of the plant. Analysis of the minerals using inductively coupled plasma atomic emission spectroscopy (ICP-OES) showed that the parts of the plant primarily consist of high levels of phosphorus, magnesium and calcium. Titration analysis of vitamin C showed that the highest concentration of the vitamin is present in the bark. High performance liquid chromatography (HPLC) analysis of vitamin E indicated that the content is the highest in the fruit, and the content of vitamin A was the highest in the leaves. Brine shrimp lethality assay (BSLA) results showed that 50% ethanol extract of the leaves is the most toxic during a 24 h treatment. Thus, the leaves and bark exhibit excellent antioxidant effects and can be potentially developed as functional food ingredients. The findings of the present study suggest further research in cell lines and in vivo.
format	Article
author	Mozhiarhasi Sandra Sagrin, Nur Fatimah Lasano, Radhiah Shukri, Nurul Shazini Ramli,
spellingShingle	Mozhiarhasi Sandra Sagrin, Nur Fatimah Lasano, Radhiah Shukri, Nurul Shazini Ramli, Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
author_facet	Mozhiarhasi Sandra Sagrin, Nur Fatimah Lasano, Radhiah Shukri, Nurul Shazini Ramli,
author_sort	Mozhiarhasi Sandra Sagrin,
title	Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_short	Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_full	Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_fullStr	Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_full_unstemmed	Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)
title_sort	antioxidant properties and toxicity assessment of the crescentia cujete extracts in brine shrimp (artemia salina)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13386/ http://journalarticle.ukm.my/13386/ http://journalarticle.ukm.my/13386/1/15%20Mozhiarhasi%20Sandra%20Sagrin.pdf
first_indexed	2023-09-18T20:04:44Z
last_indexed	2023-09-18T20:04:44Z
_version_	1777407082995122176

Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina)

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