Development of an HPLC method for formic acid analysis through peak exclusion approach

High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specif...

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Main Authors:	Hasan, Daniyal, Mahmood, Iram, Ahmad, Ishfaq, Aziz, Farah, Ahmad, Ishaq
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13622/ http://journalarticle.ukm.my/13622/ http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf

id	ukm-13622
recordtype	eprints
spelling	ukm-136222019-11-10T04:07:37Z http://journalarticle.ukm.my/13622/ Development of an HPLC method for formic acid analysis through peak exclusion approach Hasan, Daniyal Mahmood, Iram Ahmad, Ishfaq Aziz, Farah Ahmad, Ishaq High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specifically reported expensive HPLC apparatus is not available for every set of mixtures. Present study concentrated on analysis of formic acid from a mixture of methanol, formaldehyde and water. Formic acid is a basic carboxylic acid that has growing industrial and commercial uses, especially as a refrigerant. A C-18 column was utilized for the analysis by optimizing the parameters like dimensions of the stationary column, nature of mobile phase, flow rate of mobile phase and wavelength of the ultraviolet detector. Comprehendible and reproducible results for the analysis of formic acid were obtained on 25 cm long C-18 stationary column, by tuning the combined effect of water as mobile phase at a flow rate of 1 mL/min and by excluding the unnecessary peaks at 250 nm wavelength of ultraviolet detector, called peak exclusion approach. The calibration curve was found to be linear over the range of 1.25 % v/v formic acid solution in methanol up to 100 % pure formic acid. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 2.25 % and 6.83 %, respectively. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf Hasan, Daniyal and Mahmood, Iram and Ahmad, Ishfaq and Aziz, Farah and Ahmad, Ishaq (2019) Development of an HPLC method for formic acid analysis through peak exclusion approach. Sains Malaysiana, 48 (5). pp. 1011-1018. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specifically reported expensive HPLC apparatus is not available for every set of mixtures. Present study concentrated on analysis of formic acid from a mixture of methanol, formaldehyde and water. Formic acid is a basic carboxylic acid that has growing industrial and commercial uses, especially as a refrigerant. A C-18 column was utilized for the analysis by optimizing the parameters like dimensions of the stationary column, nature of mobile phase, flow rate of mobile phase and wavelength of the ultraviolet detector. Comprehendible and reproducible results for the analysis of formic acid were obtained on 25 cm long C-18 stationary column, by tuning the combined effect of water as mobile phase at a flow rate of 1 mL/min and by excluding the unnecessary peaks at 250 nm wavelength of ultraviolet detector, called peak exclusion approach. The calibration curve was found to be linear over the range of 1.25 % v/v formic acid solution in methanol up to 100 % pure formic acid. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 2.25 % and 6.83 %, respectively.
format	Article
author	Hasan, Daniyal Mahmood, Iram Ahmad, Ishfaq Aziz, Farah Ahmad, Ishaq
spellingShingle	Hasan, Daniyal Mahmood, Iram Ahmad, Ishfaq Aziz, Farah Ahmad, Ishaq Development of an HPLC method for formic acid analysis through peak exclusion approach
author_facet	Hasan, Daniyal Mahmood, Iram Ahmad, Ishfaq Aziz, Farah Ahmad, Ishaq
author_sort	Hasan, Daniyal
title	Development of an HPLC method for formic acid analysis through peak exclusion approach
title_short	Development of an HPLC method for formic acid analysis through peak exclusion approach
title_full	Development of an HPLC method for formic acid analysis through peak exclusion approach
title_fullStr	Development of an HPLC method for formic acid analysis through peak exclusion approach
title_full_unstemmed	Development of an HPLC method for formic acid analysis through peak exclusion approach
title_sort	development of an hplc method for formic acid analysis through peak exclusion approach
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13622/ http://journalarticle.ukm.my/13622/ http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf
first_indexed	2023-09-18T20:05:16Z
last_indexed	2023-09-18T20:05:16Z
_version_	1777407115849105408

Development of an HPLC method for formic acid analysis through peak exclusion approach

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