Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect

Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. A...

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Main Authors:	Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/13794/ http://journalarticle.ukm.my/13794/ http://journalarticle.ukm.my/13794/1/8.pdf

id	ukm-13794
recordtype	eprints
spelling	ukm-137942019-12-19T23:48:04Z http://journalarticle.ukm.my/13794/ Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar, Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. After primary phase separation, more LS54 (polymer) which is the system’s component itself were added into polymer-enriched phase and mixed thoroughly before thermo-induced separation step proceeded. The compositions of LS54 added into the polymer-enriched phase were 0.25, 0.5 and 0.75 g LS54/g top phase. The thermo-induced phase separation was carried out at 37°C. It was found that cutinase recovery in water-enriched phase was increased up to 5-13% with the increment of polymer concentration in the system as compared to a system without polymer addition. The optimum concentration obtained for the polymer added was 0.5 g LS54/g top phase whereby it attained 82% recovery of cutinase enzyme in water-enriched phase after thermoseparation step. Although the increment of enzyme recovery was not exceptionally high as compared to another method such as adding ligand, an affinity tag or neutral salt, still this method is applicable because of its more straightforward work, polymer recycle capability, and enzyme recovery in water phase would definitely give benefit to further downstream processing. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13794/1/8.pdf Fariza Akmal Abdul Mutalib, and Jamaliah Md Jahim, and Abdul Wahab MohammadDepartment, and Farah Diba Abu Bakar, (2018) Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect. Jurnal Kejuruteraan, 30 (2). pp. 187-192. ISSN 0128-0198 http://www.ukm.my/jkukm/volume-302-2018/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. After primary phase separation, more LS54 (polymer) which is the system’s component itself were added into polymer-enriched phase and mixed thoroughly before thermo-induced separation step proceeded. The compositions of LS54 added into the polymer-enriched phase were 0.25, 0.5 and 0.75 g LS54/g top phase. The thermo-induced phase separation was carried out at 37°C. It was found that cutinase recovery in water-enriched phase was increased up to 5-13% with the increment of polymer concentration in the system as compared to a system without polymer addition. The optimum concentration obtained for the polymer added was 0.5 g LS54/g top phase whereby it attained 82% recovery of cutinase enzyme in water-enriched phase after thermoseparation step. Although the increment of enzyme recovery was not exceptionally high as compared to another method such as adding ligand, an affinity tag or neutral salt, still this method is applicable because of its more straightforward work, polymer recycle capability, and enzyme recovery in water phase would definitely give benefit to further downstream processing.
format	Article
author	Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar,
spellingShingle	Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar, Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
author_facet	Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar,
author_sort	Fariza Akmal Abdul Mutalib,
title	Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_short	Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_full	Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_fullStr	Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_full_unstemmed	Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_sort	optimising cutinase enzyme recovery in thermo-induced phase separation of ls54/dx atps by enhanced volume exclusion effect
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/13794/ http://journalarticle.ukm.my/13794/ http://journalarticle.ukm.my/13794/1/8.pdf
first_indexed	2023-09-18T20:05:39Z
last_indexed	2023-09-18T20:05:39Z
_version_	1777407140333355008

Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect

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