Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia

Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense (Foc) has been known to hamper banana production. In Malaysia, most of the edible banana cultivars are susceptible to the fungal strain Foc tropical race 4 (Foc TR4). To date, molecular characterisation of Foc TR4 in this country r...

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Main Authors:	Wong, Clement Kiing Fook, Dzarifah Zulperi, Ganesan Vadamalai, Noor Baity Saidi, Teh, Chui Yao
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13881/ http://journalarticle.ukm.my/13881/ http://journalarticle.ukm.my/13881/1/04%20Wong%20Kiing%20Fook%20Clement.pdf

id	ukm-13881
recordtype	eprints
spelling	ukm-138812020-01-10T08:36:23Z http://journalarticle.ukm.my/13881/ Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia Wong, Clement Kiing Fook Dzarifah Zulperi, Ganesan Vadamalai, Noor Baity Saidi, Teh, Chui Yao Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense (Foc) has been known to hamper banana production. In Malaysia, most of the edible banana cultivars are susceptible to the fungal strain Foc tropical race 4 (Foc TR4). To date, molecular characterisation of Foc TR4 in this country remains elusive. Thus, this study aimed to characterise the diversity of Foc TR4 in Peninsular Malaysia based on the phylogenetic analysis of transcription elongation factor (TEF-1α) sequences. From March 2016 to December 2016, 17 isolates of Foc were isolated from nine outbreak states in Peninsular Malaysia consisting of Penang, Perak, Selangor, Negeri Sembilan, Melaka, Johor, Pahang, Kelantan and Terengganu, with disease incidence exceeding 70% in several plantations. Morphological, molecular identification using TR4 specific primers and pathogenicity assay confirmed the identity of the fungal pathogen. Pathogenicity assays indicated that the aggressiveness level varies among the isolates. Phylogenetic analyses of TEF1-α sequences showed that there was no genetic variation among the Foc isolates by clustering them into four vegetative compatibility groups (VCGs) of 01213 (TR4), 01216 (TR4), 01213/16 (TR4) and 0121 (R4). PCR amplification using VCG specific primers have further categorised 16 Foc isolates as VCG01213 and a single isolate as VCG01213/16. In addition, no correlation between TEF-1α sequences and aggressiveness of isolates could be established. Penerbit Universiti Kebangsaan Malaysia 2019-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13881/1/04%20Wong%20Kiing%20Fook%20Clement.pdf Wong, Clement Kiing Fook and Dzarifah Zulperi, and Ganesan Vadamalai, and Noor Baity Saidi, and Teh, Chui Yao (2019) Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia. Sains Malaysiana, 48 (8). pp. 1593-1600. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense (Foc) has been known to hamper banana production. In Malaysia, most of the edible banana cultivars are susceptible to the fungal strain Foc tropical race 4 (Foc TR4). To date, molecular characterisation of Foc TR4 in this country remains elusive. Thus, this study aimed to characterise the diversity of Foc TR4 in Peninsular Malaysia based on the phylogenetic analysis of transcription elongation factor (TEF-1α) sequences. From March 2016 to December 2016, 17 isolates of Foc were isolated from nine outbreak states in Peninsular Malaysia consisting of Penang, Perak, Selangor, Negeri Sembilan, Melaka, Johor, Pahang, Kelantan and Terengganu, with disease incidence exceeding 70% in several plantations. Morphological, molecular identification using TR4 specific primers and pathogenicity assay confirmed the identity of the fungal pathogen. Pathogenicity assays indicated that the aggressiveness level varies among the isolates. Phylogenetic analyses of TEF1-α sequences showed that there was no genetic variation among the Foc isolates by clustering them into four vegetative compatibility groups (VCGs) of 01213 (TR4), 01216 (TR4), 01213/16 (TR4) and 0121 (R4). PCR amplification using VCG specific primers have further categorised 16 Foc isolates as VCG01213 and a single isolate as VCG01213/16. In addition, no correlation between TEF-1α sequences and aggressiveness of isolates could be established.
format	Article
author	Wong, Clement Kiing Fook Dzarifah Zulperi, Ganesan Vadamalai, Noor Baity Saidi, Teh, Chui Yao
spellingShingle	Wong, Clement Kiing Fook Dzarifah Zulperi, Ganesan Vadamalai, Noor Baity Saidi, Teh, Chui Yao Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
author_facet	Wong, Clement Kiing Fook Dzarifah Zulperi, Ganesan Vadamalai, Noor Baity Saidi, Teh, Chui Yao
author_sort	Wong, Clement Kiing Fook
title	Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
title_short	Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
title_full	Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
title_fullStr	Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
title_full_unstemmed	Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia
title_sort	phylogenetic analysis of fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from peninsular malaysia
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13881/ http://journalarticle.ukm.my/13881/ http://journalarticle.ukm.my/13881/1/04%20Wong%20Kiing%20Fook%20Clement.pdf
first_indexed	2023-09-18T20:05:50Z
last_indexed	2023-09-18T20:05:50Z
_version_	1777407152104669184

Phylogenetic analysis of Fusarium oxysporum f. sp. cubense associated with fusarium wilt of bananas from Peninsular Malaysia

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