Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action

Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action

Melicope ptelefolia is a medicinal plant from the Rutaceae, also known as ‘tenggek burung’ in Malaysia. Traditionally, natives ingest M. ptelefolia to treat a wide range of illnesses. This study aimed to investigate the effects of M. ptelefolia aqueous extract (MPAE) on compound action potentials (C...

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Main Authors: Chia, Jasmine Siew Min, Ammar Izzati Amir Ramadan, Farihah Hanani Ghazali, Wong, Siong Jiun, Mohd Roslan Sulaiman, Tengku Azam Shah Tengku Mohamad, Mohd Khairi Hussain, Enoch Kumar Perimal
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13884/
http://journalarticle.ukm.my/13884/
http://journalarticle.ukm.my/13884/1/07%20Jasmine%20Siew%20Min%20Chia.pdf
id ukm-13884
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spelling ukm-138842020-01-10T08:40:10Z http://journalarticle.ukm.my/13884/ Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action Chia, Jasmine Siew Min Ammar Izzati Amir Ramadan, Farihah Hanani Ghazali, Wong, Siong Jiun Mohd Roslan Sulaiman, Tengku Azam Shah Tengku Mohamad, Mohd Khairi Hussain, Enoch Kumar Perimal, Melicope ptelefolia is a medicinal plant from the Rutaceae, also known as ‘tenggek burung’ in Malaysia. Traditionally, natives ingest M. ptelefolia to treat a wide range of illnesses. This study aimed to investigate the effects of M. ptelefolia aqueous extract (MPAE) on compound action potentials (CAPs) in frog sciatic nerves and its mechanism involving the opioid receptors. The effects of MPAE on CAPs in frog sciatic nerves were examined using the AD Instrument Nerve Chamber. The frog sciatic nerves were dissected from the lumbar plexus to the knee of the frog and placed in Ringer’s solution. Three treatment groups with different dosages (1, 3 and 10 mg/mL) of MPAE, including negative (vehicle) and positive control group (3 mg/mL of morphine) were tested on the frog sciatic nerves by placing them in a nerve organ chamber. Following this, the involvement of opioid receptors in the effects of MPAE on CAPs was investigated by using naloxone hydrochloride as a non-selective opioid receptor antagonist. Our results showed that the peak amplitudes of CAPs were significantly (p<0.001) reduced when treated with MPAE (3 and 10 mg/mL) in frog sciatic nerves. The MPAE-induced CAPs inhibition was reversed when pre-treated with naloxone, suggesting the involvement of the opioidergic system. These results indicated the modulatory action of MPAE on nerve conduction, which may provide important leads in the development of new therapeutic drugs through the involvement of opioid receptors. Penerbit Universiti Kebangsaan Malaysia 2019-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13884/1/07%20Jasmine%20Siew%20Min%20Chia.pdf Chia, Jasmine Siew Min and Ammar Izzati Amir Ramadan, and Farihah Hanani Ghazali, and Wong, Siong Jiun and Mohd Roslan Sulaiman, and Tengku Azam Shah Tengku Mohamad, and Mohd Khairi Hussain, and Enoch Kumar Perimal, (2019) Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action. Sains Malaysiana, 48 (8). pp. 1619-1625. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
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institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Melicope ptelefolia is a medicinal plant from the Rutaceae, also known as ‘tenggek burung’ in Malaysia. Traditionally, natives ingest M. ptelefolia to treat a wide range of illnesses. This study aimed to investigate the effects of M. ptelefolia aqueous extract (MPAE) on compound action potentials (CAPs) in frog sciatic nerves and its mechanism involving the opioid receptors. The effects of MPAE on CAPs in frog sciatic nerves were examined using the AD Instrument Nerve Chamber. The frog sciatic nerves were dissected from the lumbar plexus to the knee of the frog and placed in Ringer’s solution. Three treatment groups with different dosages (1, 3 and 10 mg/mL) of MPAE, including negative (vehicle) and positive control group (3 mg/mL of morphine) were tested on the frog sciatic nerves by placing them in a nerve organ chamber. Following this, the involvement of opioid receptors in the effects of MPAE on CAPs was investigated by using naloxone hydrochloride as a non-selective opioid receptor antagonist. Our results showed that the peak amplitudes of CAPs were significantly (p<0.001) reduced when treated with MPAE (3 and 10 mg/mL) in frog sciatic nerves. The MPAE-induced CAPs inhibition was reversed when pre-treated with naloxone, suggesting the involvement of the opioidergic system. These results indicated the modulatory action of MPAE on nerve conduction, which may provide important leads in the development of new therapeutic drugs through the involvement of opioid receptors.
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author Chia, Jasmine Siew Min
Ammar Izzati Amir Ramadan,
Farihah Hanani Ghazali,
Wong, Siong Jiun
Mohd Roslan Sulaiman,
Tengku Azam Shah Tengku Mohamad,
Mohd Khairi Hussain,
Enoch Kumar Perimal,
spellingShingle Chia, Jasmine Siew Min
Ammar Izzati Amir Ramadan,
Farihah Hanani Ghazali,
Wong, Siong Jiun
Mohd Roslan Sulaiman,
Tengku Azam Shah Tengku Mohamad,
Mohd Khairi Hussain,
Enoch Kumar Perimal,
Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
author_facet Chia, Jasmine Siew Min
Ammar Izzati Amir Ramadan,
Farihah Hanani Ghazali,
Wong, Siong Jiun
Mohd Roslan Sulaiman,
Tengku Azam Shah Tengku Mohamad,
Mohd Khairi Hussain,
Enoch Kumar Perimal,
author_sort Chia, Jasmine Siew Min
title Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
title_short Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
title_full Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
title_fullStr Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
title_full_unstemmed Inhibitory effects of Melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
title_sort inhibitory effects of melicope ptelefolia extract on compound action potentials in frog sciatic nerves and its possible mechanism of action
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13884/
http://journalarticle.ukm.my/13884/
http://journalarticle.ukm.my/13884/1/07%20Jasmine%20Siew%20Min%20Chia.pdf
first_indexed 2023-09-18T20:05:51Z
last_indexed 2023-09-18T20:05:51Z
_version_ 1777407152553459712

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