The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro

Autologous chondrocyte-seeded scaffolds have proved to be one of the most promising alternative therapies for articular cartilage defects. However, the chondrocytes have specific nutritional requirements and risk of dedifferentiation during in vitro expansion. Stichopus chloronotus aqueous extract (...

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Main Authors:	Mohd Heikal Mohd Yunus, Ahmad Nazrun Shuid, Mohd Fauzi Busra, Chua, Kien Hui, Norzana Abdul Ghafar, Rizal Abd Rani
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13890/ http://journalarticle.ukm.my/13890/ http://journalarticle.ukm.my/13890/1/13%20Mohd%20Heikal%20Mohd%20Yunus.pdf

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spelling	ukm-138902020-01-10T08:45:12Z http://journalarticle.ukm.my/13890/ The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro Mohd Heikal Mohd Yunus, Ahmad Nazrun Shuid, Mohd Fauzi Busra, Chua, Kien Hui Norzana Abdul Ghafar, Rizal Abd Rani, Autologous chondrocyte-seeded scaffolds have proved to be one of the most promising alternative therapies for articular cartilage defects. However, the chondrocytes have specific nutritional requirements and risk of dedifferentiation during in vitro expansion. Stichopus chloronotus aqueous extract (SCAE) was investigated as a medium supplement for three-dimensional (3D) collagen type I hydrogel scaffold seeded with chondrocytes to determine whether SCAE is capable of maintaining phenotype and sustaining extracellular matrix synthesis and deposition. Human osteoarthritis articular chondrocytes were isolated from the knee joint cartilage of patients underwent total knee replacement surgery. Human osteoarthritis articular chondrocytes were encapsulated in collagen type I hydrogel and cultured in basic medium with 0, 0.1 and 0.2% of SCAE. The chondrocytes in 3D culture were evaluated by means cell morphology and proliferation, quantitative phenotypic expression of collagen type I, II, aggrecan core protein and sox-9. H&E, toluidine blue staining and sulfated glycosaminoglycan (sGAG) production were analyzed after 7 days in culture. Chondrocytes cultured in 3D with various SCAE concentration appeared with polygonal morphology maintaining their chondrocytes characteristic. SCAE supplementation promoted chondrocytes proliferation and the ability of the cells to express gene encoding collagen type I decreased, whereas their ability to express collagen type II, aggrecan core protein and sox9 increased as compared to control. The cartilaginous matrices were positively stained toluidine blue concomitant with higher sGAG accumulation in SCAE-supplemented culture medium. This study shown that SCAE may be beneficial for in vitro development of 3D chondrocytes culture for use in cartilage tissue engineering therapies. Penerbit Universiti Kebangsaan Malaysia 2019-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13890/1/13%20Mohd%20Heikal%20Mohd%20Yunus.pdf Mohd Heikal Mohd Yunus, and Ahmad Nazrun Shuid, and Mohd Fauzi Busra, and Chua, Kien Hui and Norzana Abdul Ghafar, and Rizal Abd Rani, (2019) The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro. Sains Malaysiana, 48 (8). pp. 1671-1683. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Autologous chondrocyte-seeded scaffolds have proved to be one of the most promising alternative therapies for articular cartilage defects. However, the chondrocytes have specific nutritional requirements and risk of dedifferentiation during in vitro expansion. Stichopus chloronotus aqueous extract (SCAE) was investigated as a medium supplement for three-dimensional (3D) collagen type I hydrogel scaffold seeded with chondrocytes to determine whether SCAE is capable of maintaining phenotype and sustaining extracellular matrix synthesis and deposition. Human osteoarthritis articular chondrocytes were isolated from the knee joint cartilage of patients underwent total knee replacement surgery. Human osteoarthritis articular chondrocytes were encapsulated in collagen type I hydrogel and cultured in basic medium with 0, 0.1 and 0.2% of SCAE. The chondrocytes in 3D culture were evaluated by means cell morphology and proliferation, quantitative phenotypic expression of collagen type I, II, aggrecan core protein and sox-9. H&E, toluidine blue staining and sulfated glycosaminoglycan (sGAG) production were analyzed after 7 days in culture. Chondrocytes cultured in 3D with various SCAE concentration appeared with polygonal morphology maintaining their chondrocytes characteristic. SCAE supplementation promoted chondrocytes proliferation and the ability of the cells to express gene encoding collagen type I decreased, whereas their ability to express collagen type II, aggrecan core protein and sox9 increased as compared to control. The cartilaginous matrices were positively stained toluidine blue concomitant with higher sGAG accumulation in SCAE-supplemented culture medium. This study shown that SCAE may be beneficial for in vitro development of 3D chondrocytes culture for use in cartilage tissue engineering therapies.
format	Article
author	Mohd Heikal Mohd Yunus, Ahmad Nazrun Shuid, Mohd Fauzi Busra, Chua, Kien Hui Norzana Abdul Ghafar, Rizal Abd Rani,
spellingShingle	Mohd Heikal Mohd Yunus, Ahmad Nazrun Shuid, Mohd Fauzi Busra, Chua, Kien Hui Norzana Abdul Ghafar, Rizal Abd Rani, The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
author_facet	Mohd Heikal Mohd Yunus, Ahmad Nazrun Shuid, Mohd Fauzi Busra, Chua, Kien Hui Norzana Abdul Ghafar, Rizal Abd Rani,
author_sort	Mohd Heikal Mohd Yunus,
title	The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
title_short	The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
title_full	The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
title_fullStr	The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
title_full_unstemmed	The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro
title_sort	effect of stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type i hydrogel in vitro
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13890/ http://journalarticle.ukm.my/13890/ http://journalarticle.ukm.my/13890/1/13%20Mohd%20Heikal%20Mohd%20Yunus.pdf
first_indexed	2023-09-18T20:05:52Z
last_indexed	2023-09-18T20:05:52Z
_version_	1777407153430069248

The effect of Stichopus chloronotus aqueous extract on human osteoarthritis articular chondrocytes in three-dimensional collagen type I hydrogel in vitro

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