Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)

This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by...

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Main Authors:	Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N.
Format:	Article
Language:	English
Published:	Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/13962/ http://journalarticle.ukm.my/13962/ http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf

id	ukm-13962
recordtype	eprints
spelling	ukm-139622020-01-17T09:01:01Z http://journalarticle.ukm.my/13962/ Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae) Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N., This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf Wan Nurul ‘Ain W.M.N., and Nurul Wahida O., and Yaakop S., and Norefrina Shafinaz M.N., (2018) Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae). Serangga, 23 (2(SI)). pp. 183-193. ISSN 1394-5130 http://ejournal.ukm.my/serangga/issue/view/1133/showToc
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.
format	Article
author	Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N.,
spellingShingle	Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N., Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
author_facet	Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N.,
author_sort	Wan Nurul ‘Ain W.M.N.,
title	Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_short	Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_full	Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_fullStr	Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_full_unstemmed	Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_sort	morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, rhynchophorus ferrugineus (coleoptera: dryophthoridae)
publisher	Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/13962/ http://journalarticle.ukm.my/13962/ http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf
first_indexed	2023-09-18T20:06:01Z
last_indexed	2023-09-18T20:06:01Z
_version_	1777407163763785728

Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)

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