Protein and microbial determinations on worn contact lenses cleaned conventionally using the Lens2® automatic lens cleaner
The accumulation of tear film proteins as well as microbes colonization onto worn contact lenses can be eliminated conventionally by mechanical rubbing during the cleaning process. Lens2® functions in rotation manner to loosen the deposits on the contact lens and has antimicrobial coating to ke...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UKM
2008
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| Online Access: | http://journalarticle.ukm.my/1398/ http://journalarticle.ukm.my/1398/ http://journalarticle.ukm.my/1398/1/Haliza%20Abdul%20Mutalib%20et%20al..pdf |
| Summary: | The accumulation of tear film proteins as well as microbes colonization onto
worn contact lenses can be eliminated conventionally by mechanical rubbing
during the cleaning process. Lens2®
functions in rotation manner to loosen
the deposits on the contact lens and has antimicrobial coating to keep lenses
away from contamination. The objective of this study was to determine the
efficiency of Lens2®
to remove deposited protein and reduce microbial
contamination compared to conventional method. Twenty-eight subjects each
wore a pair of contact lens FDA Group 1 (Polymacon, SoftLens®
38, Bausch &
Lomb) for one month and c l eaned them us ing mul t ipurpos e solut ion
(COMPLETE
®
MoisturePLUS
TM
, Advanced Medical Optics) separately using two
different methods. The right lens was cleaned conventionally while the left lens
were cleaned using the Lens2®
The control group of thirteen subjects each .
wore a pair of contact lens for the same period and cleaned both conventionally.
These lenses and its cases were then analyzed for protein deposition using
Bichinchoninic Acid Assay (BCA) Kit (Sigma, USA) in 96-well plate. Microbial
contamination was determined by culturing the samples on nutrient agar for
bacteria and fungi and non-nutrient agar for amoeba isolation. The mean of
total protein on control lenses (17.014 ± 13.246 µg/mL) was not significantly
different from those on the Lens2®
(21.623 ± 19.127 µg/mL). There were also
low growth numbers of amoeba in each group of samples. Interestingly, there
were no growths of amoeba from all Lens2®
samples collected. There was also
low growth numbers of bacteria in each sample group whereby Lens2®
had the
lowest growth of bacteria. No growth of fungi was obtained from all samples.
The automatic lens cleaner, Lens2®
was found to be as efficient as the
conventional cleaning method. However, the Lens2®
has additional advantage
Jurnal Sains Kesihatan Malaysia 6 (1) 2008: 11-2112
because of its antimicrobial material and need shorter time in the cleaning
process as well as easy and effective. |
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