Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation

This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue rege...

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Main Authors:	Elahi, Asrar, Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf

id	ukm-14347
recordtype	eprints
spelling	ukm-143472020-03-03T08:01:52Z http://journalarticle.ukm.my/14347/ Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. Penerbit Universiti Kebangsaan Malaysia 2019-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf Elahi, Asrar and Haslina Taib, and Zurairah Berahim, and Azlina Ahmad, and Suzina Sheikh Ab Hamid, (2019) Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation. Sains Malaysiana, 48 (9). pp. 1927-1935. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation.
format	Article
author	Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid,
spellingShingle	Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
author_facet	Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid,
author_sort	Elahi, Asrar
title	Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
title_short	Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
title_full	Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
title_fullStr	Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
title_full_unstemmed	Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
title_sort	evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf
first_indexed	2023-09-18T20:06:53Z
last_indexed	2023-09-18T20:06:53Z
_version_	1777407218364186624

Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation

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