Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases

The association of human papillomavirus (HPV) with juvenile laryngeal papillomatosis has been well documented. We report two cases of juvenile laryngeal papillomatosis and correlated these cases with presence of HPV, p53 and c-erbB-2 proteins. The first case was a one-year-old male patient and the s...

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Main Authors:	Siti Aishah Md Ali, Ilina Isahak, Dahlan Sabil, Lokman Saim, Fatimah Sahlan, Abdullah Sani Mohamed
Format:	Article
Language:	English
Published:	Penerbit UKM 2006
Online Access:	http://journalarticle.ukm.my/1890/ http://journalarticle.ukm.my/1890/ http://journalarticle.ukm.my/1890/1/11._67-74_%28MH_007%29.pdf

id	ukm-1890
recordtype	eprints
spelling	ukm-18902016-12-14T06:30:23Z http://journalarticle.ukm.my/1890/ Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases Siti Aishah Md Ali, Ilina Isahak, Dahlan Sabil, Lokman Saim, Fatimah Sahlan, Abdullah Sani Mohamed, The association of human papillomavirus (HPV) with juvenile laryngeal papillomatosis has been well documented. We report two cases of juvenile laryngeal papillomatosis and correlated these cases with presence of HPV, p53 and c-erbB-2 proteins. The first case was a one-year-old male patient and the second a six-year-old female patient. Formalin-fixed paraffin-embedded biopsy specimens were tested for the presence of HPV genome by the technique of in situ hybridisation using wide spectrum and type specific biotinylated probes while the immunohistochemical expression of p53 (D07, 1:50) and c-erbB-2 (DAKO A0485, 1:300) proteins were evaluated with commercially available antibodies. Histologically the tumours in both cases showed papillary configuration of squamous papilloma. The first case detected HPV type 6, HPV type 11 and p53 protein expression while the second case showed only HPV type 6. Both cases of HPV showed positive signals confined to the nuclei in the superficial squamous epithelium. The first case showed p53 positivity seen from the basal region up to one third of the epithelium of laryngeal papillomas and the subsequent recent repeat biopsy showed the positivity of p53 had extended throughout the upper layers of the epithelium. Expression of c-erbB-2 protein was not detected in both cases. These findings were similar as in other studies where follow-up of the cases was recommended since they tend to recur Penerbit UKM 2006 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/1890/1/11._67-74_%28MH_007%29.pdf Siti Aishah Md Ali, and Ilina Isahak, and Dahlan Sabil, and Lokman Saim, and Fatimah Sahlan, and Abdullah Sani Mohamed, (2006) Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases. Medicine & Health, 1 (1). ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The association of human papillomavirus (HPV) with juvenile laryngeal papillomatosis has been well documented. We report two cases of juvenile laryngeal papillomatosis and correlated these cases with presence of HPV, p53 and c-erbB-2 proteins. The first case was a one-year-old male patient and the second a six-year-old female patient. Formalin-fixed paraffin-embedded biopsy specimens were tested for the presence of HPV genome by the technique of in situ hybridisation using wide spectrum and type specific biotinylated probes while the immunohistochemical expression of p53 (D07, 1:50) and c-erbB-2 (DAKO A0485, 1:300) proteins were evaluated with commercially available antibodies. Histologically the tumours in both cases showed papillary configuration of squamous papilloma. The first case detected HPV type 6, HPV type 11 and p53 protein expression while the second case showed only HPV type 6. Both cases of HPV showed positive signals confined to the nuclei in the superficial squamous epithelium. The first case showed p53 positivity seen from the basal region up to one third of the epithelium of laryngeal papillomas and the subsequent recent repeat biopsy showed the positivity of p53 had extended throughout the upper layers of the epithelium. Expression of c-erbB-2 protein was not detected in both cases. These findings were similar as in other studies where follow-up of the cases was recommended since they tend to recur
format	Article
author	Siti Aishah Md Ali, Ilina Isahak, Dahlan Sabil, Lokman Saim, Fatimah Sahlan, Abdullah Sani Mohamed,
spellingShingle	Siti Aishah Md Ali, Ilina Isahak, Dahlan Sabil, Lokman Saim, Fatimah Sahlan, Abdullah Sani Mohamed, Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
author_facet	Siti Aishah Md Ali, Ilina Isahak, Dahlan Sabil, Lokman Saim, Fatimah Sahlan, Abdullah Sani Mohamed,
author_sort	Siti Aishah Md Ali,
title	Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
title_short	Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
title_full	Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
title_fullStr	Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
title_full_unstemmed	Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases
title_sort	detection of human papillomavirus, p53 and c-erbb-2 protein expression in juvenile laryngeal papillomatosis: a report of 2 cases
publisher	Penerbit UKM
publishDate	2006
url	http://journalarticle.ukm.my/1890/ http://journalarticle.ukm.my/1890/ http://journalarticle.ukm.my/1890/1/11._67-74_%28MH_007%29.pdf
first_indexed	2023-09-18T19:34:38Z
last_indexed	2023-09-18T19:34:38Z
_version_	1777405189041422336

Detection of Human Papillomavirus, p53 and c-erbB-2 Protein Expression in Juvenile Laryngeal Papillomatosis: A Report of 2 Cases

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