Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
Chlorella vulgaris, a unicellular microalgae, produces many intracellular phytochemicals namely carotenoids, tocopherols, ubiquinone and protein. Skin ageing which is induced by oxidative stress involves decreased extracellular matrix synthesis and increased expression of enzymes that degrade the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UKM
2010
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| Online Access: | http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/1/04MS102_112.pdf |
| Summary: | Chlorella vulgaris, a unicellular microalgae, produces many intracellular phytochemicals
namely carotenoids, tocopherols, ubiquinone and protein. Skin ageing which is
induced by oxidative stress involves decreased extracellular matrix synthesis and increased
expression of enzymes that degrade the collagenous matrix. The objective of
this study was to determine the effect of C. vulgaris on the expression of genes encoded
for collagen (COL) and matrix metalloproteinases (MMPs) which are involved in
skin ageing. Human diploid fibroblasts (HDFs) were obtained from circumcision foreskin
of 8-12 year-old boys. HDFs were cultured into 3 groups: untreated control cells,
cells with stress-induced premature senescence (SIPS; cells were induced with H2O2
at passage 6 for 2 weeks) and SIPS treated with C. vulgaris (prolonged C. vulgaris
treatment started at passage 4 and combined treatment with H2O2 at passage 6 for 2
weeks). Senescence-associated ß-galactosidase (SA ß-gal) was determined using
senescent cells histochemical staining kit (Sigma, USA). Expression of COLI, COLIII,
COLIV, MMPI, MMPII and MMPIII genes was quantitatively analysed with real-time
RT-PCR method (iScript™ One Step real-time PCR with SYBR® Green; Biorad).
HDFs treated with H2O2 (SIPS) exhibited senescent morphological features of flattening
and enlarged with increased expression of SA ß-gal (p<0.05). Gene expression
analysis showed COLI was downregulated in SIPS and SIPS treated with C. vulgaris
(p<0.05) while COLIII decreased in SIPS and increased in SIPS treated with C. vulgaris
(p<0.05). Expression of MMPI was increased in SIPS and SIPS treated with C. vulgaris
(p<0.05) indicating its synergistic effect with H2O2 treatment. In conclusion, in
skin ageing, COLI and COLIII genes were downregulated while MMPI was upregulated.
C. vulgaris modulated the expression of COL and MMP genes by downregulating
COLI and upregulating COLIII and MMPI but it did not exert anti-ageing effect. |
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