Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?

Chlorella vulgaris, a unicellular microalgae, produces many intracellular phytochemicals namely carotenoids, tocopherols, ubiquinone and protein. Skin ageing which is induced by oxidative stress involves decreased extracellular matrix synthesis and increased expression of enzymes that degrade the...

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Main Authors:	Loke CY, Nur Hidayah MS, Mohd Fadhli MF, Teo SK, Nor Hidayah AG, Yasmin Anum MY, Suzana M
Format:	Article
Language:	English
Published:	Penerbit UKM 2010
Online Access:	http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/1/04MS102_112.pdf

id	ukm-2040
recordtype	eprints
spelling	ukm-20402016-12-14T06:30:44Z http://journalarticle.ukm.my/2040/ Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing? Loke CY, Nur Hidayah MS, Mohd Fadhli MF, Teo SK, Nor Hidayah AG, Yasmin Anum MY, Suzana M, Chlorella vulgaris, a unicellular microalgae, produces many intracellular phytochemicals namely carotenoids, tocopherols, ubiquinone and protein. Skin ageing which is induced by oxidative stress involves decreased extracellular matrix synthesis and increased expression of enzymes that degrade the collagenous matrix. The objective of this study was to determine the effect of C. vulgaris on the expression of genes encoded for collagen (COL) and matrix metalloproteinases (MMPs) which are involved in skin ageing. Human diploid fibroblasts (HDFs) were obtained from circumcision foreskin of 8-12 year-old boys. HDFs were cultured into 3 groups: untreated control cells, cells with stress-induced premature senescence (SIPS; cells were induced with H2O2 at passage 6 for 2 weeks) and SIPS treated with C. vulgaris (prolonged C. vulgaris treatment started at passage 4 and combined treatment with H2O2 at passage 6 for 2 weeks). Senescence-associated ß-galactosidase (SA ß-gal) was determined using senescent cells histochemical staining kit (Sigma, USA). Expression of COLI, COLIII, COLIV, MMPI, MMPII and MMPIII genes was quantitatively analysed with real-time RT-PCR method (iScript™ One Step real-time PCR with SYBR® Green; Biorad). HDFs treated with H2O2 (SIPS) exhibited senescent morphological features of flattening and enlarged with increased expression of SA ß-gal (p<0.05). Gene expression analysis showed COLI was downregulated in SIPS and SIPS treated with C. vulgaris (p<0.05) while COLIII decreased in SIPS and increased in SIPS treated with C. vulgaris (p<0.05). Expression of MMPI was increased in SIPS and SIPS treated with C. vulgaris (p<0.05) indicating its synergistic effect with H2O2 treatment. In conclusion, in skin ageing, COLI and COLIII genes were downregulated while MMPI was upregulated. C. vulgaris modulated the expression of COL and MMP genes by downregulating COLI and upregulating COLIII and MMPI but it did not exert anti-ageing effect. Penerbit UKM 2010 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2040/1/04MS102_112.pdf Loke CY, and Nur Hidayah MS, and Mohd Fadhli MF, and Teo SK, and Nor Hidayah AG, and Yasmin Anum MY, and Suzana M, (2010) Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing? Medicine & Health, 5 (1). pp. 1-12. ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Chlorella vulgaris, a unicellular microalgae, produces many intracellular phytochemicals namely carotenoids, tocopherols, ubiquinone and protein. Skin ageing which is induced by oxidative stress involves decreased extracellular matrix synthesis and increased expression of enzymes that degrade the collagenous matrix. The objective of this study was to determine the effect of C. vulgaris on the expression of genes encoded for collagen (COL) and matrix metalloproteinases (MMPs) which are involved in skin ageing. Human diploid fibroblasts (HDFs) were obtained from circumcision foreskin of 8-12 year-old boys. HDFs were cultured into 3 groups: untreated control cells, cells with stress-induced premature senescence (SIPS; cells were induced with H2O2 at passage 6 for 2 weeks) and SIPS treated with C. vulgaris (prolonged C. vulgaris treatment started at passage 4 and combined treatment with H2O2 at passage 6 for 2 weeks). Senescence-associated ß-galactosidase (SA ß-gal) was determined using senescent cells histochemical staining kit (Sigma, USA). Expression of COLI, COLIII, COLIV, MMPI, MMPII and MMPIII genes was quantitatively analysed with real-time RT-PCR method (iScript™ One Step real-time PCR with SYBR® Green; Biorad). HDFs treated with H2O2 (SIPS) exhibited senescent morphological features of flattening and enlarged with increased expression of SA ß-gal (p<0.05). Gene expression analysis showed COLI was downregulated in SIPS and SIPS treated with C. vulgaris (p<0.05) while COLIII decreased in SIPS and increased in SIPS treated with C. vulgaris (p<0.05). Expression of MMPI was increased in SIPS and SIPS treated with C. vulgaris (p<0.05) indicating its synergistic effect with H2O2 treatment. In conclusion, in skin ageing, COLI and COLIII genes were downregulated while MMPI was upregulated. C. vulgaris modulated the expression of COL and MMP genes by downregulating COLI and upregulating COLIII and MMPI but it did not exert anti-ageing effect.
format	Article
author	Loke CY, Nur Hidayah MS, Mohd Fadhli MF, Teo SK, Nor Hidayah AG, Yasmin Anum MY, Suzana M,
spellingShingle	Loke CY, Nur Hidayah MS, Mohd Fadhli MF, Teo SK, Nor Hidayah AG, Yasmin Anum MY, Suzana M, Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
author_facet	Loke CY, Nur Hidayah MS, Mohd Fadhli MF, Teo SK, Nor Hidayah AG, Yasmin Anum MY, Suzana M,
author_sort	Loke CY,
title	Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
title_short	Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
title_full	Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
title_fullStr	Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
title_full_unstemmed	Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?
title_sort	does chlorella vulgaris modulate the expression of col and mmp genes in skin ageing?
publisher	Penerbit UKM
publishDate	2010
url	http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/ http://journalarticle.ukm.my/2040/1/04MS102_112.pdf
first_indexed	2023-09-18T19:35:01Z
last_indexed	2023-09-18T19:35:01Z
_version_	1777405213037035520

Does chlorella vulgaris modulate the expression of COL and MMP Genes in skin ageing?

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