An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds

The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentra...

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Main Authors:	Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2394/ http://journalarticle.ukm.my/2394/ http://journalarticle.ukm.my/2394/1/09_Fatima.pdf

id	ukm-2394
recordtype	eprints
spelling	ukm-23942016-12-14T06:31:29Z http://journalarticle.ukm.my/2394/ An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman, The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentrations (0.5, 1.0, 1.5, and 2.0 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy-acetic acid (2,4-D) alone or in combination with 0.5 mg/L of 6-benzylamino purine (BAP). For milk clotting activity, about 50 or 100 μL extracts of seed callus was mixed with 2 mL 50% milk held at 55°C for 5 and 10 min until milk clotting occurred. The results showed that NAA alone or in combination with BAP gave a higher callusing percentage (80 to100%) compared to the other plant growth regulators at the same concentrations. When an auxin was supplied in combination with BAP, a significant increase in callusing percentage or degree of callusing was observed. The time required for callus to be developed was shortened and the quality of the induced callus improved. An increase in callus growth in low sucrose (10 g/L) concentration was found be comparable (88%) to high sucrose concentration (30 g/L; 60%). Crude extracts obtained from S. dubium callus were shown to exhibit milk clotting activity. Universiti Kebangsaan Malaysia 2011-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2394/1/09_Fatima.pdf Fatima Musbah Abbas, and ElBushra ElSheikh ElNur, and Normah Mohd Noor Eisa Algaali, and Zainon Mohd Ali, and Roohaida Othman, (2011) An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds. Sains Malaysiana, 40 (4). pp. 339-343. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentrations (0.5, 1.0, 1.5, and 2.0 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy-acetic acid (2,4-D) alone or in combination with 0.5 mg/L of 6-benzylamino purine (BAP). For milk clotting activity, about 50 or 100 μL extracts of seed callus was mixed with 2 mL 50% milk held at 55°C for 5 and 10 min until milk clotting occurred. The results showed that NAA alone or in combination with BAP gave a higher callusing percentage (80 to100%) compared to the other plant growth regulators at the same concentrations. When an auxin was supplied in combination with BAP, a significant increase in callusing percentage or degree of callusing was observed. The time required for callus to be developed was shortened and the quality of the induced callus improved. An increase in callus growth in low sucrose (10 g/L) concentration was found be comparable (88%) to high sucrose concentration (30 g/L; 60%). Crude extracts obtained from S. dubium callus were shown to exhibit milk clotting activity.
format	Article
author	Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman,
spellingShingle	Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman, An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
author_facet	Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman,
author_sort	Fatima Musbah Abbas,
title	An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_short	An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_full	An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_fullStr	An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_full_unstemmed	An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_sort	effective protocol for callus induction with milk clotting activity from solanum dubiumseeds
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2394/ http://journalarticle.ukm.my/2394/ http://journalarticle.ukm.my/2394/1/09_Fatima.pdf
first_indexed	2023-09-18T19:35:59Z
last_indexed	2023-09-18T19:35:59Z
_version_	1777405274200473600

An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds

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