Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process

B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral acti...

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Main Authors:	Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf

id	ukm-2639
recordtype	eprints
spelling	ukm-26392016-12-14T06:32:13Z http://journalarticle.ukm.my/2639/ Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral activity against ascites carcinoma cells, and a hypocholesterolemic activity that inhibits cAMP phosphodiesterase, as well as having anti-HIV properties. A cost effective recovery and purification of surfactin from fermentation broth using a two-step ultrafiltration (UF) process has been developed in order to reduce the cost of surfactin production. In this study, competitive adsorption of surfactin and proteins at the air-water interface was studied using surface pressure measurements. Small volumes of bovine serum albumin (BSA) and β-casein solutions were added to the air-water interface on a Langmuir trough and allowed to stabilise before the addition of surfactin to the subphase. Contrasting interfacial behaviour of proteins was observed with β-casein showing faster initial adsorption compared to BSA. On introduction of surfactin both proteins were displaced but a longer time were taken to displace β-casein. Overall the results showed surfactin were highly surface-active by forming a β-sheet structure at the air-water interface after reaching its critical micelle concentration (CMC) and were effective in removing both protein films, which can be explained following the orogenic mechanism. Results showed that the two-step UF process was effective to achieve high purity and fully functional surfactin. Universiti Kebangsaan Malaysia 2011-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf Mohd. Hafez Mohd Isa, and Richard Frazier .A, and Paula Jauregi, (2011) Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process. Sains Malaysiana, 40 (9). pp. 1043-1048. ISSN 0126-6039 http:www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral activity against ascites carcinoma cells, and a hypocholesterolemic activity that inhibits cAMP phosphodiesterase, as well as having anti-HIV properties. A cost effective recovery and purification of surfactin from fermentation broth using a two-step ultrafiltration (UF) process has been developed in order to reduce the cost of surfactin production. In this study, competitive adsorption of surfactin and proteins at the air-water interface was studied using surface pressure measurements. Small volumes of bovine serum albumin (BSA) and β-casein solutions were added to the air-water interface on a Langmuir trough and allowed to stabilise before the addition of surfactin to the subphase. Contrasting interfacial behaviour of proteins was observed with β-casein showing faster initial adsorption compared to BSA. On introduction of surfactin both proteins were displaced but a longer time were taken to displace β-casein. Overall the results showed surfactin were highly surface-active by forming a β-sheet structure at the air-water interface after reaching its critical micelle concentration (CMC) and were effective in removing both protein films, which can be explained following the orogenic mechanism. Results showed that the two-step UF process was effective to achieve high purity and fully functional surfactin.
format	Article
author	Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi,
spellingShingle	Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
author_facet	Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi,
author_sort	Mohd. Hafez Mohd Isa,
title	Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
title_short	Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
title_full	Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
title_fullStr	Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
title_full_unstemmed	Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
title_sort	surface activity of surfactin recovered and purified from fermentation broth using a two-step ultrafiltration (uf) process
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf
first_indexed	2023-09-18T19:36:36Z
last_indexed	2023-09-18T19:36:36Z
_version_	1777405313165557760

Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process

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