Detection of toluene degradation in bacteria isolated from oil contaminated soils

Toluene (C7H8) a hydrocarbon in crude oil, is a common contaminant in soil and groundwater. In this study, the ability to degrade toluene was investigated from twelve bacteria isolates which were isolated from soil contaminated with oil. Out of 12 bacterial isolates tested, most of Pseudomonas sp. s...

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Main Authors:	Ainon Hamzah, Arezoo Tavakoli, Amir Rabu
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2932/ http://journalarticle.ukm.my/2932/ http://journalarticle.ukm.my/2932/1/04_Ainon_Hamzah.pdf

id	ukm-2932
recordtype	eprints
spelling	ukm-29322016-12-14T06:33:05Z http://journalarticle.ukm.my/2932/ Detection of toluene degradation in bacteria isolated from oil contaminated soils Ainon Hamzah, Arezoo Tavakoli, Amir Rabu, Toluene (C7H8) a hydrocarbon in crude oil, is a common contaminant in soil and groundwater. In this study, the ability to degrade toluene was investigated from twelve bacteria isolates which were isolated from soil contaminated with oil. Out of 12 bacterial isolates tested, most of Pseudomonas sp. showed the capability to grow in 1 mM of toluene compared with other isolates on the third day of incubation. Based on enzyme assays towards toluene monooxygenase, Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were shown to have the highest ability to degrade toluene. The toluene monoxygenase activity was analysed by using two calorimetric methods, Horseradish peroxidase (HRP) and indole-indigo. Both of the methods measured the production of catechol by the enzymatic reaction of toluene monooxygenase. In the HRP assay, the highest enzyme activity was 0.274 U/mL, exhibited by Pseudomonas aeruginosa UKMP-14T. However, for indole-indigo assay, Bacillus cereus UKMP-6G produced the highest enzyme activity of 0.291 U/ml. Results from both experiments showed that Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were able to degrade toluene. Universiti Kebangsaan Malaysia 2011-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2932/1/04_Ainon_Hamzah.pdf Ainon Hamzah, and Arezoo Tavakoli, and Amir Rabu, (2011) Detection of toluene degradation in bacteria isolated from oil contaminated soils. Sains Malaysiana, 40 (11). pp. 1231-1235. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Toluene (C7H8) a hydrocarbon in crude oil, is a common contaminant in soil and groundwater. In this study, the ability to degrade toluene was investigated from twelve bacteria isolates which were isolated from soil contaminated with oil. Out of 12 bacterial isolates tested, most of Pseudomonas sp. showed the capability to grow in 1 mM of toluene compared with other isolates on the third day of incubation. Based on enzyme assays towards toluene monooxygenase, Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were shown to have the highest ability to degrade toluene. The toluene monoxygenase activity was analysed by using two calorimetric methods, Horseradish peroxidase (HRP) and indole-indigo. Both of the methods measured the production of catechol by the enzymatic reaction of toluene monooxygenase. In the HRP assay, the highest enzyme activity was 0.274 U/mL, exhibited by Pseudomonas aeruginosa UKMP-14T. However, for indole-indigo assay, Bacillus cereus UKMP-6G produced the highest enzyme activity of 0.291 U/ml. Results from both experiments showed that Pseudomonas aeruginosa UKMP-14T and Bacillus cereus UKMP-6G were able to degrade toluene.
format	Article
author	Ainon Hamzah, Arezoo Tavakoli, Amir Rabu,
spellingShingle	Ainon Hamzah, Arezoo Tavakoli, Amir Rabu, Detection of toluene degradation in bacteria isolated from oil contaminated soils
author_facet	Ainon Hamzah, Arezoo Tavakoli, Amir Rabu,
author_sort	Ainon Hamzah,
title	Detection of toluene degradation in bacteria isolated from oil contaminated soils
title_short	Detection of toluene degradation in bacteria isolated from oil contaminated soils
title_full	Detection of toluene degradation in bacteria isolated from oil contaminated soils
title_fullStr	Detection of toluene degradation in bacteria isolated from oil contaminated soils
title_full_unstemmed	Detection of toluene degradation in bacteria isolated from oil contaminated soils
title_sort	detection of toluene degradation in bacteria isolated from oil contaminated soils
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2932/ http://journalarticle.ukm.my/2932/ http://journalarticle.ukm.my/2932/1/04_Ainon_Hamzah.pdf
first_indexed	2023-09-18T19:37:23Z
last_indexed	2023-09-18T19:37:23Z
_version_	1777405362402492416

Detection of toluene degradation in bacteria isolated from oil contaminated soils

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