An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients

Recent association studies have described genetic variants among type 2 diabetes mellitus (T2DM) and their related traits. Gestational diabetes mellitus (GDM) is pathophysiologically similar to T2DM and may share genetic susceptibility.However, genetic susceptibility within GDM in our own populatio...

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Main Authors:	Nor Khatijah Mohd Aris, Nor Azlin Mohamed Ismai, Zaleha Abdul Mahdy, Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Halizah Haji Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah, Syed Zulkifli Syed Zakaria
Format:	Article
Language:	English
Published:	UKM Medical Molecular biology Institute, UMBI 2011
Online Access:	http://journalarticle.ukm.my/3063/ http://journalarticle.ukm.my/3063/ http://journalarticle.ukm.my/3063/1/2011-1-1.pdf

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spelling	ukm-30632016-12-14T06:33:28Z http://journalarticle.ukm.my/3063/ An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients Nor Khatijah Mohd Aris, Nor Azlin Mohamed Ismai, Zaleha Abdul Mahdy, Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Halizah Haji Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah, Syed Zulkifli Syed Zakaria, Recent association studies have described genetic variants among type 2 diabetes mellitus (T2DM) and their related traits. Gestational diabetes mellitus (GDM) is pathophysiologically similar to T2DM and may share genetic susceptibility.However, genetic susceptibility within GDM in our own population is still not yet explored. This study was to determine the association of GDM genetic variants in the Malaysian population.We genotyped 384 T2DM related SNPs among 174 cases of GDM and 114 controls of pregnant women using Illumina’s Golden Gate genotyping assay. In this case-control study, a custom of 384-SNP plex of 236 candidate genes was designed using the Illumina’s Assay Design Tool. The data analysis showed 12 SNPs had a significant association with GDM among Malaysians with p values 0.002 to 0.048 with their respective odd ratios. The SNPs rs7754840, rs10946398, rs9465871, rs7756992, rs6823091, rs7935082,rs237889, rs7903146, rs7961581 were significant under additive model while rs10811661, rs1016472, rs2270031 were associated with GDM under recessive model. Three SNPs namely rs7935082, rs1016472 and rs2270031 had reduced risk towards GDM while another nine SNPs which were rs7754840, rs10946398, rs9465871, rs7756992,rs10811661, rs6823091, rs237889, rs7903146 and rs7961581 had increased risk as much 1.75 to 2.62 times. Twelve genetic variants of T2DM were replicated in the SNP profiling among Malaysians GDM. Thus with a more significant result in a bigger sample, SNP screening is potentially a useful method in predicting the risk of gestational diabetes mellitus. UKM Medical Molecular biology Institute, UMBI 2011 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3063/1/2011-1-1.pdf Nor Khatijah Mohd Aris, and Nor Azlin Mohamed Ismai, and Zaleha Abdul Mahdy, and Shuhaila Ahmad, and Norzilawati Mohd Naim, and Harlina Halizah Haji Siraj, and Rohana Jaafar, and Shareena Ishak, and Roslan Harun, and Rahman Jamal, and Wan Zurinah Wan Ngah, and Syed Zulkifli Syed Zakaria, (2011) An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients. Asia-Pacific Journal of Molecular Medicine, 1 . pp. 1-8. ISSN 2232-0326 http://www.umbi.ukm.my/umbi/index.php?option=com_content&task=view&id=388&Itemid=72
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Recent association studies have described genetic variants among type 2 diabetes mellitus (T2DM) and their related traits. Gestational diabetes mellitus (GDM) is pathophysiologically similar to T2DM and may share genetic susceptibility.However, genetic susceptibility within GDM in our own population is still not yet explored. This study was to determine the association of GDM genetic variants in the Malaysian population.We genotyped 384 T2DM related SNPs among 174 cases of GDM and 114 controls of pregnant women using Illumina’s Golden Gate genotyping assay. In this case-control study, a custom of 384-SNP plex of 236 candidate genes was designed using the Illumina’s Assay Design Tool. The data analysis showed 12 SNPs had a significant association with GDM among Malaysians with p values 0.002 to 0.048 with their respective odd ratios. The SNPs rs7754840, rs10946398, rs9465871, rs7756992, rs6823091, rs7935082,rs237889, rs7903146, rs7961581 were significant under additive model while rs10811661, rs1016472, rs2270031 were associated with GDM under recessive model. Three SNPs namely rs7935082, rs1016472 and rs2270031 had reduced risk towards GDM while another nine SNPs which were rs7754840, rs10946398, rs9465871, rs7756992,rs10811661, rs6823091, rs237889, rs7903146 and rs7961581 had increased risk as much 1.75 to 2.62 times. Twelve genetic variants of T2DM were replicated in the SNP profiling among Malaysians GDM. Thus with a more significant result in a bigger sample, SNP screening is potentially a useful method in predicting the risk of gestational diabetes mellitus.
format	Article
author	Nor Khatijah Mohd Aris, Nor Azlin Mohamed Ismai, Zaleha Abdul Mahdy, Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Halizah Haji Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah, Syed Zulkifli Syed Zakaria,
spellingShingle	Nor Khatijah Mohd Aris, Nor Azlin Mohamed Ismai, Zaleha Abdul Mahdy, Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Halizah Haji Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah, Syed Zulkifli Syed Zakaria, An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
author_facet	Nor Khatijah Mohd Aris, Nor Azlin Mohamed Ismai, Zaleha Abdul Mahdy, Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Halizah Haji Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah, Syed Zulkifli Syed Zakaria,
author_sort	Nor Khatijah Mohd Aris,
title	An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
title_short	An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
title_full	An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
title_fullStr	An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
title_full_unstemmed	An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients
title_sort	analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of malaysian patients
publisher	UKM Medical Molecular biology Institute, UMBI
publishDate	2011
url	http://journalarticle.ukm.my/3063/ http://journalarticle.ukm.my/3063/ http://journalarticle.ukm.my/3063/1/2011-1-1.pdf
first_indexed	2023-09-18T19:37:46Z
last_indexed	2023-09-18T19:37:46Z
_version_	1777405385969238016

An analysis of targeted single nucleotide polymorphisms for the risk prediction of gestational diabetes mellitus in a cohort of Malaysian patients

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