Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))

The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slid...

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Main Authors:	Sharifa, A.A, Jamaludin, J, Kiong, L.S, Chia, L.A, Osman, K
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/3227/ http://journalarticle.ukm.my/3227/ http://journalarticle.ukm.my/3227/1/04%2520A.A.%2520Sharifa.pdf

id	ukm-3227
recordtype	eprints
spelling	ukm-32272016-12-14T06:34:00Z http://journalarticle.ukm.my/3227/ Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing)) Sharifa, A.A Jamaludin, J Kiong, L.S Chia, L.A Osman, K The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/ml - 250 μg/ml) significantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals significantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e 100 μg/mL and 150 μg/mL respectively (p< 0.05). However the IC50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate. Universiti Kebangsaan Malaysia 2012-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3227/1/04%2520A.A.%2520Sharifa.pdf Sharifa, A.A and Jamaludin, J and Kiong, L.S and Chia, L.A and Osman, K (2012) Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing)). Sains Malaysiana, 41 (1). pp. 33-39. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/ml - 250 μg/ml) significantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals significantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e 100 μg/mL and 150 μg/mL respectively (p< 0.05). However the IC50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate.
format	Article
author	Sharifa, A.A Jamaludin, J Kiong, L.S Chia, L.A Osman, K
spellingShingle	Sharifa, A.A Jamaludin, J Kiong, L.S Chia, L.A Osman, K Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
author_facet	Sharifa, A.A Jamaludin, J Kiong, L.S Chia, L.A Osman, K
author_sort	Sharifa, A.A
title	Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
title_short	Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
title_full	Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
title_fullStr	Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
title_full_unstemmed	Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))
title_sort	anti-urolithiatic terpenoid compound from plantago major linn. (ekor anjing)(aktiviti anti-urolitiatik sebatian “terpenoid” daripada plantago major linn. (ekor anjing))
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/3227/ http://journalarticle.ukm.my/3227/ http://journalarticle.ukm.my/3227/1/04%2520A.A.%2520Sharifa.pdf
first_indexed	2023-09-18T19:38:14Z
last_indexed	2023-09-18T19:38:14Z
_version_	1777405415113359360

Anti-urolithiatic terpenoid compound from plantago major linn. (Ekor Anjing)(Aktiviti anti-urolitiatik sebatian “terpenoid” daripada Plantago major Linn. (Ekor Anjing))

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