Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)

This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khe...

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Main Authors:	Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/3232/ http://journalarticle.ukm.my/3232/ http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf

id	ukm-3232
recordtype	eprints
spelling	ukm-32322016-12-14T06:34:01Z http://journalarticle.ukm.my/3232/ Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat) Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khefji Sour crude oil. Although it grew at pH between 5.0 and 7.0, it grew best at pH 5.5. T. virens UKMP-1M grew at temperatures between 25°C and 35°C, with its highest growth at 30°C. Aeration by agitation at 200 rpm was shown to yield the greatest biomass. Peptone at concentration of 1.5% (w/v) was determined to be a better nitrogen source than urea, potassium nitrate (KNO3), yeast extract, ammonium sulphate ((NH4)2SO4) and ammonium chloride (NH4Cl). Addition of 1% (v/v) crude oil to the MSM medium led to higher biomass production than the addition of 3%, 5%, 7% and 10% (v/v) crude oil. The result also revealed that 40% of total petroleum hydrocarbon (TPH), 100% of pristane and 74% of phytane compounds were degraded after 9 days of incubation at optimal physical and nutrient parameters. Universiti Kebangsaan Malaysia 2012-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf Ainon Hamzah, and Mazni Abu Zain, and Othman Omar, and Sahidan Senafi, (2012) Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat). Sains Malaysiana, 41 (1). pp. 71-79. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khefji Sour crude oil. Although it grew at pH between 5.0 and 7.0, it grew best at pH 5.5. T. virens UKMP-1M grew at temperatures between 25°C and 35°C, with its highest growth at 30°C. Aeration by agitation at 200 rpm was shown to yield the greatest biomass. Peptone at concentration of 1.5% (w/v) was determined to be a better nitrogen source than urea, potassium nitrate (KNO3), yeast extract, ammonium sulphate ((NH4)2SO4) and ammonium chloride (NH4Cl). Addition of 1% (v/v) crude oil to the MSM medium led to higher biomass production than the addition of 3%, 5%, 7% and 10% (v/v) crude oil. The result also revealed that 40% of total petroleum hydrocarbon (TPH), 100% of pristane and 74% of phytane compounds were degraded after 9 days of incubation at optimal physical and nutrient parameters.
format	Article
author	Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi,
spellingShingle	Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi, Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
author_facet	Ainon Hamzah, Mazni Abu Zain, Othman Omar, Sahidan Senafi,
author_sort	Ainon Hamzah,
title	Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
title_short	Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
title_full	Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
title_fullStr	Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
title_full_unstemmed	Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)
title_sort	optimal physical and nutrient parameters for growth of trichoderma virens ukmp-1m for heavy crude oil degradation (pengoptimuman parameter fizikal dan nutrien bagi pertumbuhan trichoderma virens ukmp-1m untuk degradasi minyak mentah berat)
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/3232/ http://journalarticle.ukm.my/3232/ http://journalarticle.ukm.my/3232/1/09_Ainon_Hamzah.pdf
first_indexed	2023-09-18T19:38:14Z
last_indexed	2023-09-18T19:38:14Z
_version_	1777405415838973952

Optimal physical and nutrient parameters for growth of trichoderma virens UKMP-1M for heavy crude oil degradation (Pengoptimuman Parameter Fizikal dan nutrien bagi pertumbuhan trichoderma virens UKMP-1M untuk degradasi minyak mentah berat)

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