Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)

Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)

A method of PCR-restriction fragment length polymorphism (RFLP) has been utilized to differentiate the mitochondrial genes of pork and wild boar meat (Sus scrofa). The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of these two meats. The amplificatio...

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Main Authors: Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Safiyyah Shahimi, Norhayati Yaakob, Norrakiah Abdullah Sani, Aminah Abdullah, Abdul Salam Babji, Maaruf Abd. Ghani
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2012
Online Access:http://journalarticle.ukm.my/3291/
http://journalarticle.ukm.my/3291/
http://journalarticle.ukm.my/3291/1/07%2520Sahilah.pdf
id ukm-3291
recordtype eprints
spelling ukm-32912016-12-14T06:34:14Z http://journalarticle.ukm.my/3291/ Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP) Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Safiyyah Shahimi, Norhayati Yaakob, Norrakiah Abdullah Sani, Aminah Abdullah, Abdul Salam Babji, Maaruf Abd. Ghani, A method of PCR-restriction fragment length polymorphism (RFLP) has been utilized to differentiate the mitochondrial genes of pork and wild boar meat (Sus scrofa). The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of these two meats. The amplification product of pork and wild boar using mt-12S rRNA gene successfully produced a single band with molecular size of 456 bp. Three restriction endonucleases (AluI, HindIII and BsaJI) were used to restrict the amplification products of the mitochondrial genes. The restriction enzymes of AluI and BsaJI were identified as potential restriction endonucleases to differentiate those meats. HindIII enzyme was unable to restrict the PCR product of both meats. The genetic differences within the cyt b gene among the two meats were successfully confirmed by PCR-RFLP analysis Universiti Kebangsaan Malaysia 2012-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3291/1/07%2520Sahilah.pdf Sahilah Abd. Mutalib, and Wan Sakeenah Wan Nazri, and Safiyyah Shahimi, and Norhayati Yaakob, and Norrakiah Abdullah Sani, and Aminah Abdullah, and Abdul Salam Babji, and Maaruf Abd. Ghani, (2012) Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP). Sains Malaysiana, 41 (2). pp. 199-204. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description A method of PCR-restriction fragment length polymorphism (RFLP) has been utilized to differentiate the mitochondrial genes of pork and wild boar meat (Sus scrofa). The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of these two meats. The amplification product of pork and wild boar using mt-12S rRNA gene successfully produced a single band with molecular size of 456 bp. Three restriction endonucleases (AluI, HindIII and BsaJI) were used to restrict the amplification products of the mitochondrial genes. The restriction enzymes of AluI and BsaJI were identified as potential restriction endonucleases to differentiate those meats. HindIII enzyme was unable to restrict the PCR product of both meats. The genetic differences within the cyt b gene among the two meats were successfully confirmed by PCR-RFLP analysis
format Article
author Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Safiyyah Shahimi,
Norhayati Yaakob,
Norrakiah Abdullah Sani,
Aminah Abdullah,
Abdul Salam Babji,
Maaruf Abd. Ghani,
spellingShingle Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Safiyyah Shahimi,
Norhayati Yaakob,
Norrakiah Abdullah Sani,
Aminah Abdullah,
Abdul Salam Babji,
Maaruf Abd. Ghani,
Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
author_facet Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Safiyyah Shahimi,
Norhayati Yaakob,
Norrakiah Abdullah Sani,
Aminah Abdullah,
Abdul Salam Babji,
Maaruf Abd. Ghani,
author_sort Sahilah Abd. Mutalib,
title Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
title_short Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
title_full Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
title_fullStr Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
title_full_unstemmed Comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)(Perbandingan di antara daging babi dan babi hutan (Sus scrofa) dengan menggunakan analisis tindak balas rantaian Polymerase-Polimorfisme panjang cebisan pemotongan (PCR-RFLP)
title_sort comparison between pork and wild boar meat (sus scrofa) by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp)(perbandingan di antara daging babi dan babi hutan (sus scrofa) dengan menggunakan analisis tindak balas rantaian polymerase-polimorfisme panjang cebisan pemotongan (pcr-rflp)
publisher Universiti Kebangsaan Malaysia
publishDate 2012
url http://journalarticle.ukm.my/3291/
http://journalarticle.ukm.my/3291/
http://journalarticle.ukm.my/3291/1/07%2520Sahilah.pdf
first_indexed 2023-09-18T19:38:25Z
last_indexed 2023-09-18T19:38:25Z
_version_ 1777405427304103936

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