Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves

Response surface methodology (RSM) in conjunction with central composite rotatable design (CCRD) was performed in the present study to optimize the extraction parameters for assessing maximum yield of total phenolic content (TPC) from henna (Lawsonia inermis) leaves. The range of the independent var...

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Main Authors:	D.B. Uma, C.W. Ho, W.M. Wan Aida
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/334/ http://journalarticle.ukm.my/334/ http://journalarticle.ukm.my/334/1/

id	ukm-334
recordtype	eprints
spelling	ukm-3342016-12-14T06:27:05Z http://journalarticle.ukm.my/334/ Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves D.B. Uma, C.W. Ho, W.M. Wan Aida, Response surface methodology (RSM) in conjunction with central composite rotatable design (CCRD) was performed in the present study to optimize the extraction parameters for assessing maximum yield of total phenolic content (TPC) from henna (Lawsonia inermis) leaves. The range of the independent variables, namely acetone concentration (20-90%, v/v), extraction time (10-90 minutes) and extraction temperature (25-45°C) were identified by a first set of single factor experiments. The actual values of the independent variables coded at five levels were selected based on the results of single factor experiments. The optimum conditions for extraction of TPC were found to be at acetone concentration 48.07%, extraction time 73.78 minutes and extraction temperature 39.57°C. Under these optimized conditions, the experimental maximum yield of TPC was 7203.74 mg GAE/100g DW, which was in close agreement with predicted values, thus indicating the suitability of the models developed and the success of RSM in optimizing the extraction conditions. Universiti Kebangsaan Malaysia 2010-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/334/1/ D.B. Uma, and C.W. Ho, and W.M. Wan Aida, (2010) Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves. Sains Malaysiana, 39 (1). pp. 119-128. ISSN 0126-6039 http://www.ukm.my/~jsm/contents.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Response surface methodology (RSM) in conjunction with central composite rotatable design (CCRD) was performed in the present study to optimize the extraction parameters for assessing maximum yield of total phenolic content (TPC) from henna (Lawsonia inermis) leaves. The range of the independent variables, namely acetone concentration (20-90%, v/v), extraction time (10-90 minutes) and extraction temperature (25-45°C) were identified by a first set of single factor experiments. The actual values of the independent variables coded at five levels were selected based on the results of single factor experiments. The optimum conditions for extraction of TPC were found to be at acetone concentration 48.07%, extraction time 73.78 minutes and extraction temperature 39.57°C. Under these optimized conditions, the experimental maximum yield of TPC was 7203.74 mg GAE/100g DW, which was in close agreement with predicted values, thus indicating the suitability of the models developed and the success of RSM in optimizing the extraction conditions.
format	Article
author	D.B. Uma, C.W. Ho, W.M. Wan Aida,
spellingShingle	D.B. Uma, C.W. Ho, W.M. Wan Aida, Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
author_facet	D.B. Uma, C.W. Ho, W.M. Wan Aida,
author_sort	D.B. Uma,
title	Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
title_short	Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
title_full	Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
title_fullStr	Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
title_full_unstemmed	Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves
title_sort	optimization of extraction parameters of total phenolic compounds from henna (lawsonia inermis) leaves
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/334/ http://journalarticle.ukm.my/334/ http://journalarticle.ukm.my/334/1/
first_indexed	2023-09-18T19:30:37Z
last_indexed	2023-09-18T19:30:37Z
_version_	1777404936351383552

Optimization of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) Leaves

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