Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization

Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investi...

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Main Authors:	Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim
Format:	Article
Language:	English
Published:	Penerbit UKM 2011
Online Access:	http://journalarticle.ukm.my/3596/ http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf

id	ukm-3596
recordtype	eprints
spelling	ukm-35962016-12-14T06:34:54Z http://journalarticle.ukm.my/3596/ Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim, Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investigate the physiological characteristics of survivability during axenization. Six Acanthamoeba strains from three clinical isolates (HSB 1, HKL 48 and HKL 95) and three environmental isolates (PHS 2, PHS 11 and PHS 15) were used in this study. Axenization test was done by treating cysts with hydrochloric acid (3%) and Page saline containing Gentamicin (100 μg/ml). Cysts were then cultured into PYG enrich media, incubated at 30oC and the presence and proliferation of trophozoites of Acanthamoeba were observed. This study showed that PHS 15, HSB 1, HKL 48 and HKL 95 could be axenized but they have poor proliferation rate in PYG enrich media. The result showed that the difference between both clinical and environmental isolates was observed in two strains; PHS 2 and PHS 11. This indicates that there is a possibility that the physiological traits of strains from both isolates are the same and strains from the environment are able to show the pathogenic potential and capable of causing infection to human. Penerbit UKM 2011 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf Nurul Farhana Jufri, and Anisah Nordin, and Mohamed Kamel Abd Ghani, and Yusof Suboh, and Noraina Abd Rahim, (2011) Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization. Jurnal Sains Kesihatan Malaysia, 9 (1). pp. 1-3. ISSN 1675-8161
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investigate the physiological characteristics of survivability during axenization. Six Acanthamoeba strains from three clinical isolates (HSB 1, HKL 48 and HKL 95) and three environmental isolates (PHS 2, PHS 11 and PHS 15) were used in this study. Axenization test was done by treating cysts with hydrochloric acid (3%) and Page saline containing Gentamicin (100 μg/ml). Cysts were then cultured into PYG enrich media, incubated at 30oC and the presence and proliferation of trophozoites of Acanthamoeba were observed. This study showed that PHS 15, HSB 1, HKL 48 and HKL 95 could be axenized but they have poor proliferation rate in PYG enrich media. The result showed that the difference between both clinical and environmental isolates was observed in two strains; PHS 2 and PHS 11. This indicates that there is a possibility that the physiological traits of strains from both isolates are the same and strains from the environment are able to show the pathogenic potential and capable of causing infection to human.
format	Article
author	Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim,
spellingShingle	Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim, Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
author_facet	Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim,
author_sort	Nurul Farhana Jufri,
title	Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_short	Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_full	Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_fullStr	Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_full_unstemmed	Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_sort	survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
publisher	Penerbit UKM
publishDate	2011
url	http://journalarticle.ukm.my/3596/ http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf
first_indexed	2023-09-18T19:39:13Z
last_indexed	2023-09-18T19:39:13Z
_version_	1777405477537185792

Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization

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