Fabrikasi slaid mikroatur cDNA lates calcaficer

DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression of various model systems performed. DNA microarray performance is measured by parameters like spot density, spot characteristics (morphology, probe density and hybri...

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Main Authors:	Khoo, Choon Kiat, Adura Mohd-Adnan, Kua, Beng Chu, Abdul Munir Abdul Murad
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2009
Online Access:	http://journalarticle.ukm.my/36/ http://journalarticle.ukm.my/36/ http://journalarticle.ukm.my/36/1/

id	ukm-36
recordtype	eprints
spelling	ukm-362016-12-14T06:26:13Z http://journalarticle.ukm.my/36/ Fabrikasi slaid mikroatur cDNA lates calcaficer Khoo, Choon Kiat Adura Mohd-Adnan, Kua, Beng Chu Abdul Munir Abdul Murad, DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression of various model systems performed. DNA microarray performance is measured by parameters like spot density, spot characteristics (morphology, probe density and hybridised intensity), background, specificity and sensitivity. Using the GeneTac™G3 arrayer, we fabricated the Asian seabass (Lates calacrifer) cDNA microarray, comprising 1920 selected cDNAs clones sourced from spleen and liver cDNA libraries, and spotted in duplicate. Putative functions encoded by these clones include immunity, stress response, metabolism, transport, transcription and translation. Negative controls such as oligonucleotide (A)50, and several Saccharomyces cerevisiae and Escherichia coli genes as well as positive controls such as a serial dilution of L. calcarifer genomic DNA and cDNA were also spotted onto the glass slide. It was observed that the probes concentration ranging from 150-250 μg/mL and the type of positive and negative control employed produced quality slide. In addition, the overnight drying of the spotted slides and slide rehydrating produced morphologically even and round DNA spots. Slide quality controls employed demonstrated the functionality of the fabricated slides in profiling the L. calcarifer transcriptome response toward Cryptocaryon irritans infection. Universiti Kebangsaan Malaysia 2009-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/36/1/ Khoo, Choon Kiat and Adura Mohd-Adnan, and Kua, Beng Chu and Abdul Munir Abdul Murad, (2009) Fabrikasi slaid mikroatur cDNA lates calcaficer. Sains Malaysiana, 38 (4). pp. 609-617. ISSN 0126-6039 http://www.ukm.my/~jsm/kandungan.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression of various model systems performed. DNA microarray performance is measured by parameters like spot density, spot characteristics (morphology, probe density and hybridised intensity), background, specificity and sensitivity. Using the GeneTac™G3 arrayer, we fabricated the Asian seabass (Lates calacrifer) cDNA microarray, comprising 1920 selected cDNAs clones sourced from spleen and liver cDNA libraries, and spotted in duplicate. Putative functions encoded by these clones include immunity, stress response, metabolism, transport, transcription and translation. Negative controls such as oligonucleotide (A)50, and several Saccharomyces cerevisiae and Escherichia coli genes as well as positive controls such as a serial dilution of L. calcarifer genomic DNA and cDNA were also spotted onto the glass slide. It was observed that the probes concentration ranging from 150-250 μg/mL and the type of positive and negative control employed produced quality slide. In addition, the overnight drying of the spotted slides and slide rehydrating produced morphologically even and round DNA spots. Slide quality controls employed demonstrated the functionality of the fabricated slides in profiling the L. calcarifer transcriptome response toward Cryptocaryon irritans infection.
format	Article
author	Khoo, Choon Kiat Adura Mohd-Adnan, Kua, Beng Chu Abdul Munir Abdul Murad,
spellingShingle	Khoo, Choon Kiat Adura Mohd-Adnan, Kua, Beng Chu Abdul Munir Abdul Murad, Fabrikasi slaid mikroatur cDNA lates calcaficer
author_facet	Khoo, Choon Kiat Adura Mohd-Adnan, Kua, Beng Chu Abdul Munir Abdul Murad,
author_sort	Khoo, Choon Kiat
title	Fabrikasi slaid mikroatur cDNA lates calcaficer
title_short	Fabrikasi slaid mikroatur cDNA lates calcaficer
title_full	Fabrikasi slaid mikroatur cDNA lates calcaficer
title_fullStr	Fabrikasi slaid mikroatur cDNA lates calcaficer
title_full_unstemmed	Fabrikasi slaid mikroatur cDNA lates calcaficer
title_sort	fabrikasi slaid mikroatur cdna lates calcaficer
publisher	Universiti Kebangsaan Malaysia
publishDate	2009
url	http://journalarticle.ukm.my/36/ http://journalarticle.ukm.my/36/ http://journalarticle.ukm.my/36/1/
first_indexed	2023-09-18T19:01:05Z
last_indexed	2023-09-18T19:01:05Z
_version_	1777403078163562496

Fabrikasi slaid mikroatur cDNA lates calcaficer

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