The development of a suitable cabbage tissue culture system for gene transformation studies

Experiments were carried out to establish optimum culture condition and to identify the most responsive hypocotyls segments to regenerate shoots in the presence of slight callus for the local cabbage cultivar, Brassica oleracea L. var capitata subvar. kkcross. Results of the study showed that only t...

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Main Authors:	Latifah Amin, Mohd Azib Salleh, Abdul Karim Abdul Ghani, Mohd Nazir Basiran
Format:	Article
Published:	Universiti Kebangsaan Malaysia 2006
Online Access:	http://journalarticle.ukm.my/3976/ http://journalarticle.ukm.my/3976/

id	ukm-3976
recordtype	eprints
spelling	ukm-39762012-03-28T01:05:32Z http://journalarticle.ukm.my/3976/ The development of a suitable cabbage tissue culture system for gene transformation studies Latifah Amin, Mohd Azib Salleh, Abdul Karim Abdul Ghani, Mohd Nazir Basiran, Experiments were carried out to establish optimum culture condition and to identify the most responsive hypocotyls segments to regenerate shoots in the presence of slight callus for the local cabbage cultivar, Brassica oleracea L. var capitata subvar. kkcross. Results of the study showed that only the upper segment of the hypocotyls (1 cm and 2 cm from both shoot apex) excised from in vitro seedlings, regenerate shoots while the segment nearest to the apex (1 cm) regenerate both shoots and roots. Optimum regeneration was obtained when the seedlings' age were 3 weeks old as compared to younger or older explants. Types of culture vessels did not exhibit any significant effect on the hypocotyls culture. Multiple shoots with the presence of slight healthy callus (0.5 - 2.0mm diameter) were successfully regenerated at a frequency of 42 and 50% respectively from hypocotyls sections cultured on a basal Murashige and Skoog (MS) medium supplemented with 3-lndole acetic acid (1AA) [1 µg/L (w/v)], kinetin [2 µg/L(w/v)] and casein hydrolysate [500 µg/L(w/ v)] (designated as Ms1 medium) and B5 medium supplemented with adenine sulphate [40 mg/L(w/v)], glutamine (6mM) and 2, 4,5-trichlorophenoxyacetic acid [2,4,5- T (0.1 mg/L( w/v)] (designated as cs23 medium). Clonal propagation from axillary buds was induced on a basal MS medium supplemented with low concentration of kinetin [0.05-0.2mg/L(w/v)]. Plantlets with complete roots were successfully transferred into a mixture of soil and vermiculate (1: 1) and grown into normal mature cabbage plants in the glasshouse. Universiti Kebangsaan Malaysia 2006-07 Article PeerReviewed Latifah Amin, and Mohd Azib Salleh, and Abdul Karim Abdul Ghani, and Mohd Nazir Basiran, (2006) The development of a suitable cabbage tissue culture system for gene transformation studies. Sains Malaysiana, 35 (1). pp. 61-66. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol35num1_2006/vol35num1_06page61-66.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
description	Experiments were carried out to establish optimum culture condition and to identify the most responsive hypocotyls segments to regenerate shoots in the presence of slight callus for the local cabbage cultivar, Brassica oleracea L. var capitata subvar. kkcross. Results of the study showed that only the upper segment of the hypocotyls (1 cm and 2 cm from both shoot apex) excised from in vitro seedlings, regenerate shoots while the segment nearest to the apex (1 cm) regenerate both shoots and roots. Optimum regeneration was obtained when the seedlings' age were 3 weeks old as compared to younger or older explants. Types of culture vessels did not exhibit any significant effect on the hypocotyls culture. Multiple shoots with the presence of slight healthy callus (0.5 - 2.0mm diameter) were successfully regenerated at a frequency of 42 and 50% respectively from hypocotyls sections cultured on a basal Murashige and Skoog (MS) medium supplemented with 3-lndole acetic acid (1AA) [1 µg/L (w/v)], kinetin [2 µg/L(w/v)] and casein hydrolysate [500 µg/L(w/ v)] (designated as Ms1 medium) and B5 medium supplemented with adenine sulphate [40 mg/L(w/v)], glutamine (6mM) and 2, 4,5-trichlorophenoxyacetic acid [2,4,5- T (0.1 mg/L( w/v)] (designated as cs23 medium). Clonal propagation from axillary buds was induced on a basal MS medium supplemented with low concentration of kinetin [0.05-0.2mg/L(w/v)]. Plantlets with complete roots were successfully transferred into a mixture of soil and vermiculate (1: 1) and grown into normal mature cabbage plants in the glasshouse.
format	Article
author	Latifah Amin, Mohd Azib Salleh, Abdul Karim Abdul Ghani, Mohd Nazir Basiran,
spellingShingle	Latifah Amin, Mohd Azib Salleh, Abdul Karim Abdul Ghani, Mohd Nazir Basiran, The development of a suitable cabbage tissue culture system for gene transformation studies
author_facet	Latifah Amin, Mohd Azib Salleh, Abdul Karim Abdul Ghani, Mohd Nazir Basiran,
author_sort	Latifah Amin,
title	The development of a suitable cabbage tissue culture system for gene transformation studies
title_short	The development of a suitable cabbage tissue culture system for gene transformation studies
title_full	The development of a suitable cabbage tissue culture system for gene transformation studies
title_fullStr	The development of a suitable cabbage tissue culture system for gene transformation studies
title_full_unstemmed	The development of a suitable cabbage tissue culture system for gene transformation studies
title_sort	development of a suitable cabbage tissue culture system for gene transformation studies
publisher	Universiti Kebangsaan Malaysia
publishDate	2006
url	http://journalarticle.ukm.my/3976/ http://journalarticle.ukm.my/3976/
first_indexed	2023-09-18T19:40:18Z
last_indexed	2023-09-18T19:40:18Z
_version_	1777405545140977664

The development of a suitable cabbage tissue culture system for gene transformation studies

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