Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver

Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (S...

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Main Authors:	Chong, Ching June, Lee, Hui Wen, Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin, Noor Embi, Hasidah Mohd Sidek
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/5418/ http://journalarticle.ukm.my/5418/ http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf

id	ukm-5418
recordtype	eprints
spelling	ukm-54182016-12-14T06:38:23Z http://journalarticle.ukm.my/5418/ Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway. Universiti Kebangsaan Malaysia 2012-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf Chong, Ching June and Lee, Hui Wen and Halimah Abdullah Sani, and Jalifah Latip, and Jualang Azlan Gansau, and Lee, Ping Chin and Noor Embi, and Hasidah Mohd Sidek, (2012) Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver. Sains Malaysiana, 41 (8). pp. 969-975. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway.
format	Article
author	Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek,
spellingShingle	Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
author_facet	Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek,
author_sort	Chong, Ching June
title	Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
title_short	Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
title_full	Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
title_fullStr	Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
title_full_unstemmed	Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver
title_sort	hypoglycemic effects of gynura procumbensfractions on streptozotocin-induced diabetic rats involve phosphorylation of gsk3β (ser-9) in liver
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/5418/ http://journalarticle.ukm.my/5418/ http://journalarticle.ukm.my/5418/1/05%2520Chong%2520Ching.pdf
first_indexed	2023-09-18T19:44:03Z
last_indexed	2023-09-18T19:44:03Z
_version_	1777405781600108544

Hypoglycemic effects of gynura procumbensFractions on streptozotocin-induced diabetic rats involve phosphorylation of GSK3β (Ser-9) in liver

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