Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV

The objective of this study was to develop a method for the determination of diuron (3-(3,4-dichlorophenyl)-1,1-dimethyl urea) residue in crude palm oil (CPO) and crude palm kernel oil (CPKO) matrices. The method involves the extraction of the herbicide from the oil matrix using low temperature prec...

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Main Authors:	Mohd. Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan, Yew Ai, Halimah Muhamad
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/5582/ http://journalarticle.ukm.my/5582/ http://journalarticle.ukm.my/5582/1/15%2520Mohd%2520Izwarie%2520.pdf

id	ukm-5582
recordtype	eprints
spelling	ukm-55822016-12-14T06:38:53Z http://journalarticle.ukm.my/5582/ Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV Mohd. Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan, Yew Ai Halimah Muhamad, The objective of this study was to develop a method for the determination of diuron (3-(3,4-dichlorophenyl)-1,1-dimethyl urea) residue in crude palm oil (CPO) and crude palm kernel oil (CPKO) matrices. The method involves the extraction of the herbicide from the oil matrix using low temperature precipitation and solid phase extraction techniques, detected by high performance liquid chromatography-ultra violet (HPLC-UV). The HPLC separation was carried out on an AscentisTM RP-Amide column and elution with acetonitrile (solvent A) and water-methanol (2:1, v/v) (solvent B) as a suitable solvent system, at ratio of 4:6 (v/v). The optimum volume of acetonitrile for the extraction of diuron was 30 mL and 4 mL was obtained as the optimum volume of the solvent for elution analyte through the SPE cartridge. A linear correlation was obtained for the concentration of diuron from 0.05–1.0 μg mL-1 with a correlation coefficient of 0.99. The recovery of diuron from CPO was 83.2–101.4% with a relative standard deviation of 1.4–9.9% and 79.4–87.9% with relative standard deviation of 0.9–5.6% for CPKO. The method detection limit and limit of quantification obtained were 0.018 μg g-1 and 0.058 μg g-1, respectively. The method was used to determine diuron residues in palm oil from different refineries situated at different locations throughout Malaysia Universiti Kebangsaan Malaysia 2012-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5582/1/15%2520Mohd%2520Izwarie%2520.pdf Mohd. Izwarie Ramli, and Zuriati Zakaria, and Ismail Sahid, and Tan, Yew Ai and Halimah Muhamad, (2012) Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV. Sains Malaysiana, 41 (11). pp. 1451-1459. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The objective of this study was to develop a method for the determination of diuron (3-(3,4-dichlorophenyl)-1,1-dimethyl urea) residue in crude palm oil (CPO) and crude palm kernel oil (CPKO) matrices. The method involves the extraction of the herbicide from the oil matrix using low temperature precipitation and solid phase extraction techniques, detected by high performance liquid chromatography-ultra violet (HPLC-UV). The HPLC separation was carried out on an AscentisTM RP-Amide column and elution with acetonitrile (solvent A) and water-methanol (2:1, v/v) (solvent B) as a suitable solvent system, at ratio of 4:6 (v/v). The optimum volume of acetonitrile for the extraction of diuron was 30 mL and 4 mL was obtained as the optimum volume of the solvent for elution analyte through the SPE cartridge. A linear correlation was obtained for the concentration of diuron from 0.05–1.0 μg mL-1 with a correlation coefficient of 0.99. The recovery of diuron from CPO was 83.2–101.4% with a relative standard deviation of 1.4–9.9% and 79.4–87.9% with relative standard deviation of 0.9–5.6% for CPKO. The method detection limit and limit of quantification obtained were 0.018 μg g-1 and 0.058 μg g-1, respectively. The method was used to determine diuron residues in palm oil from different refineries situated at different locations throughout Malaysia
format	Article
author	Mohd. Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan, Yew Ai Halimah Muhamad,
spellingShingle	Mohd. Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan, Yew Ai Halimah Muhamad, Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
author_facet	Mohd. Izwarie Ramli, Zuriati Zakaria, Ismail Sahid, Tan, Yew Ai Halimah Muhamad,
author_sort	Mohd. Izwarie Ramli,
title	Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
title_short	Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
title_full	Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
title_fullStr	Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
title_full_unstemmed	Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV
title_sort	determination of herbicide diuron levels in palm oil matrices using hplc-uv
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/5582/ http://journalarticle.ukm.my/5582/ http://journalarticle.ukm.my/5582/1/15%2520Mohd%2520Izwarie%2520.pdf
first_indexed	2023-09-18T19:44:33Z
last_indexed	2023-09-18T19:44:33Z
_version_	1777405813078360064

Determination of Herbicide Diuron levels in palm oil matrices using HPLC-UV

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