In vitro and in vivo anti-plasmodial activities of gynura procumbens

Gynura procumbens, locally known in Malaysia as Sambung Nyawa is a medicinal plant belonging to the Asteraceae (Compositae) family. G. procumbens have been traditionally used by the local and indigenous populations to treat an array of ailments ranging from skin conditions and fever to kidney diseas...

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Main Authors:	Visalini Vejanan, Jalifah Latip, Lee, Ping Chin, Noor Embi, Hasidah Mohd Sidek
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/5670/ http://journalarticle.ukm.my/5670/ http://journalarticle.ukm.my/5670/1/06%2520Visalini.pdf

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spelling	ukm-56702016-12-14T06:39:08Z http://journalarticle.ukm.my/5670/ In vitro and in vivo anti-plasmodial activities of gynura procumbens Visalini Vejanan, Jalifah Latip, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, Gynura procumbens, locally known in Malaysia as Sambung Nyawa is a medicinal plant belonging to the Asteraceae (Compositae) family. G. procumbens have been traditionally used by the local and indigenous populations to treat an array of ailments ranging from skin conditions and fever to kidney disease, inflammation and diabetes. In the present investigation, aqueous and ethanol extracts of procumbens were evaluated for anti-plasmodial activities in vitro and in vivo. Survival of two chloroquine-sensitive strains of malarial parasites; rodent Plasmodium berghei NK65 and human Plasmodium falciparum 3D7 was determined following incubations in vitro with extracts. Based on parasite lactate dehydrogenase (pLDH) assay, both extracts were shown to inhibit parasite proliferation to varying degrees. The aqueous extract was more potent than the ethanol extract at suppressing growth of both parasites in vitro; each displaying IC50 values of 12.40 ± 6.02 and 14.38 ± 7.53 μg/mL towards P. berghei NK65; and 25.69 ± 4.34 and 42.23 ± 7.19 μg/mL towards falciparum 3D7, respectively. The aqueous extract was found to be selective for falciparum (Selectivity Index 64.30). Four-day suppressive tests in ICR mice showed dose-dependent chemo-suppressive activities of both plant extracts tested towards berghei NK65. Daily intra-peritoneal injections of the aqueous extract of G. procumbens at 25, 50 or 100 mg/kg for four consecutive days showed chemo-suppression of 50.42 ± 3.17, 65.95 ± 5.48 and 81.92 ± 3.07%, respectively. At the same dosages, the ethanol plant extract resulted in 44.97 ± 3.44, 55.21 ± 3.87 and 64.44 ± 4.05% chemo-suppression respectively. At 250 mg/kg/day, only the aqueous plant extract gave >90% chemo-suppression (93.06 ± 5.46%). Treatment of P. berghei-infected mice with extracts improved the median survival time compared with non-treated infected mice. This represents the first report showing anti-plasmodial activity of procumbens. Universiti Kebangsaan Malaysia 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5670/1/06%2520Visalini.pdf Visalini Vejanan, and Jalifah Latip, and Lee, Ping Chin and Noor Embi, and Hasidah Mohd Sidek, (2012) In vitro and in vivo anti-plasmodial activities of gynura procumbens. Sains Malaysiana, 41 (12). pp. 1535-1542. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Gynura procumbens, locally known in Malaysia as Sambung Nyawa is a medicinal plant belonging to the Asteraceae (Compositae) family. G. procumbens have been traditionally used by the local and indigenous populations to treat an array of ailments ranging from skin conditions and fever to kidney disease, inflammation and diabetes. In the present investigation, aqueous and ethanol extracts of procumbens were evaluated for anti-plasmodial activities in vitro and in vivo. Survival of two chloroquine-sensitive strains of malarial parasites; rodent Plasmodium berghei NK65 and human Plasmodium falciparum 3D7 was determined following incubations in vitro with extracts. Based on parasite lactate dehydrogenase (pLDH) assay, both extracts were shown to inhibit parasite proliferation to varying degrees. The aqueous extract was more potent than the ethanol extract at suppressing growth of both parasites in vitro; each displaying IC50 values of 12.40 ± 6.02 and 14.38 ± 7.53 μg/mL towards P. berghei NK65; and 25.69 ± 4.34 and 42.23 ± 7.19 μg/mL towards falciparum 3D7, respectively. The aqueous extract was found to be selective for falciparum (Selectivity Index 64.30). Four-day suppressive tests in ICR mice showed dose-dependent chemo-suppressive activities of both plant extracts tested towards berghei NK65. Daily intra-peritoneal injections of the aqueous extract of G. procumbens at 25, 50 or 100 mg/kg for four consecutive days showed chemo-suppression of 50.42 ± 3.17, 65.95 ± 5.48 and 81.92 ± 3.07%, respectively. At the same dosages, the ethanol plant extract resulted in 44.97 ± 3.44, 55.21 ± 3.87 and 64.44 ± 4.05% chemo-suppression respectively. At 250 mg/kg/day, only the aqueous plant extract gave >90% chemo-suppression (93.06 ± 5.46%). Treatment of P. berghei-infected mice with extracts improved the median survival time compared with non-treated infected mice. This represents the first report showing anti-plasmodial activity of procumbens.
format	Article
author	Visalini Vejanan, Jalifah Latip, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek,
spellingShingle	Visalini Vejanan, Jalifah Latip, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek, In vitro and in vivo anti-plasmodial activities of gynura procumbens
author_facet	Visalini Vejanan, Jalifah Latip, Lee, Ping Chin Noor Embi, Hasidah Mohd Sidek,
author_sort	Visalini Vejanan,
title	In vitro and in vivo anti-plasmodial activities of gynura procumbens
title_short	In vitro and in vivo anti-plasmodial activities of gynura procumbens
title_full	In vitro and in vivo anti-plasmodial activities of gynura procumbens
title_fullStr	In vitro and in vivo anti-plasmodial activities of gynura procumbens
title_full_unstemmed	In vitro and in vivo anti-plasmodial activities of gynura procumbens
title_sort	in vitro and in vivo anti-plasmodial activities of gynura procumbens
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/5670/ http://journalarticle.ukm.my/5670/ http://journalarticle.ukm.my/5670/1/06%2520Visalini.pdf
first_indexed	2023-09-18T19:44:48Z
last_indexed	2023-09-18T19:44:48Z
_version_	1777405828789174272

In vitro and in vivo anti-plasmodial activities of gynura procumbens

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