Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee

An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with...

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Main Authors:	Zailina, A.M, Aminah, A, Mohd Ambar, Y
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/5895/ http://journalarticle.ukm.my/5895/ http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf

id	ukm-5895
recordtype	eprints
spelling	ukm-58952016-12-14T06:39:47Z http://journalarticle.ukm.my/5895/ Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee Zailina, A.M Aminah, A Mohd Ambar, Y An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with and without evaporation steps) were evaluated. Identification and quantitation was conducted by high performance liquid chromatography with photo-diode-array (HPLC-DAD) at different wavelengths; 230 nm, 245 nm and 290 nm; and by time of flight mass spectrometry (LC-MS-TOF). Extraction with acetonitrile (without evaporation with nitrogen) showed recovery ranging from 105% to 113% (± <10%) for HPLC-DAD at 245 nm and 93% to 102% (± <2.5%) for LC-MS-TOF. Chromatogram separation was best achieved with mobile phase consisted of water (0.1% formic acid) and acetonitrile (0.1% formic acid) with gradient elution within 20 min. Thus, the results indicated that extraction using acetonitrile without evaporation step was the most efficient technique for determination of PDE5 inhibitors in premix coffee. Universiti Kebangsaan Malaysia 2013-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf Zailina, A.M and Aminah, A and Mohd Ambar, Y (2013) Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee. Sains Malaysiana, 42 (2). pp. 135-142. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with and without evaporation steps) were evaluated. Identification and quantitation was conducted by high performance liquid chromatography with photo-diode-array (HPLC-DAD) at different wavelengths; 230 nm, 245 nm and 290 nm; and by time of flight mass spectrometry (LC-MS-TOF). Extraction with acetonitrile (without evaporation with nitrogen) showed recovery ranging from 105% to 113% (± <10%) for HPLC-DAD at 245 nm and 93% to 102% (± <2.5%) for LC-MS-TOF. Chromatogram separation was best achieved with mobile phase consisted of water (0.1% formic acid) and acetonitrile (0.1% formic acid) with gradient elution within 20 min. Thus, the results indicated that extraction using acetonitrile without evaporation step was the most efficient technique for determination of PDE5 inhibitors in premix coffee.
format	Article
author	Zailina, A.M Aminah, A Mohd Ambar, Y
spellingShingle	Zailina, A.M Aminah, A Mohd Ambar, Y Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
author_facet	Zailina, A.M Aminah, A Mohd Ambar, Y
author_sort	Zailina, A.M
title	Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_short	Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_full	Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_fullStr	Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_full_unstemmed	Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_sort	optimization of extraction methods for determination of phosphodiesterase-5 (pde5) inhibitors in premix coffee
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/5895/ http://journalarticle.ukm.my/5895/ http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf
first_indexed	2023-09-18T19:45:27Z
last_indexed	2023-09-18T19:45:27Z
_version_	1777405869831487488

Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee

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