Antimicrobial activity of nigella sativa seed extract

Pathogenic bacterial infections have become a major health problem worldwide. New antimicrobial agents are urgently needed to overcome this problem. In this study, antibacterial activity of Nigella sativa seed extract against some pathogenic bacterial strains (Streptococcus pyogene, Pseudomonas aeru...

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Main Authors:	Nor’ Aishah Hasan, Mohd Zaini Nawahwi, Haslinda Ab Malek
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/5896/ http://journalarticle.ukm.my/5896/ http://journalarticle.ukm.my/5896/1/04%2520NorAishah.pdf

id	ukm-5896
recordtype	eprints
spelling	ukm-58962016-12-14T06:39:47Z http://journalarticle.ukm.my/5896/ Antimicrobial activity of nigella sativa seed extract Nor’ Aishah Hasan, Mohd Zaini Nawahwi, Haslinda Ab Malek, Pathogenic bacterial infections have become a major health problem worldwide. New antimicrobial agents are urgently needed to overcome this problem. In this study, antibacterial activity of Nigella sativa seed extract against some pathogenic bacterial strains (Streptococcus pyogene, Pseudomonas aeruginosa, Klebseilla pneumoniae and Proteus vulgaris) was evaluated. Methanol extract at the concentration of 100 mg/mL had a remarkable sensitivity towards all tested bacteria in this study. Klebseila pneumonia and Proteus vulgaris showed resistance against aqueous extract at 20 mg/mL. Methanol extract of Nigella sativa exhibited significant antibacterial activity at the concentration of 50 mg/mL (p ≤ 0.01) against Streptococcus pyogenes with a greater inhibition zone of 19 mm, while a 15 mm zone of inhibition was observed in Pseudomonas aeruginosa, pneumonia and Proteus vulgaris. Kruskal Wallis analysis showed that both aqueous and methanol extract of black seed exhibited a greater inhibition on Gram positive bacteria (Streptococcus pyogenes) compared with Gram negative bacteria (Pseudomonas aeruginosa, Klebseilla pneumoniae and Proteus vulgaris). Our study also showed that species, strains and concentrations of Nigella sativa extract are some of the factors that may influence the sensitivity of the tested bacteria. A significant correlation was observed between zone of inhibition and concentration of extract. Universiti Kebangsaan Malaysia 2013-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5896/1/04%2520NorAishah.pdf Nor’ Aishah Hasan, and Mohd Zaini Nawahwi, and Haslinda Ab Malek, (2013) Antimicrobial activity of nigella sativa seed extract. Sains Malaysiana, 42 (2). pp. 143-147. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Pathogenic bacterial infections have become a major health problem worldwide. New antimicrobial agents are urgently needed to overcome this problem. In this study, antibacterial activity of Nigella sativa seed extract against some pathogenic bacterial strains (Streptococcus pyogene, Pseudomonas aeruginosa, Klebseilla pneumoniae and Proteus vulgaris) was evaluated. Methanol extract at the concentration of 100 mg/mL had a remarkable sensitivity towards all tested bacteria in this study. Klebseila pneumonia and Proteus vulgaris showed resistance against aqueous extract at 20 mg/mL. Methanol extract of Nigella sativa exhibited significant antibacterial activity at the concentration of 50 mg/mL (p ≤ 0.01) against Streptococcus pyogenes with a greater inhibition zone of 19 mm, while a 15 mm zone of inhibition was observed in Pseudomonas aeruginosa, pneumonia and Proteus vulgaris. Kruskal Wallis analysis showed that both aqueous and methanol extract of black seed exhibited a greater inhibition on Gram positive bacteria (Streptococcus pyogenes) compared with Gram negative bacteria (Pseudomonas aeruginosa, Klebseilla pneumoniae and Proteus vulgaris). Our study also showed that species, strains and concentrations of Nigella sativa extract are some of the factors that may influence the sensitivity of the tested bacteria. A significant correlation was observed between zone of inhibition and concentration of extract.
format	Article
author	Nor’ Aishah Hasan, Mohd Zaini Nawahwi, Haslinda Ab Malek,
spellingShingle	Nor’ Aishah Hasan, Mohd Zaini Nawahwi, Haslinda Ab Malek, Antimicrobial activity of nigella sativa seed extract
author_facet	Nor’ Aishah Hasan, Mohd Zaini Nawahwi, Haslinda Ab Malek,
author_sort	Nor’ Aishah Hasan,
title	Antimicrobial activity of nigella sativa seed extract
title_short	Antimicrobial activity of nigella sativa seed extract
title_full	Antimicrobial activity of nigella sativa seed extract
title_fullStr	Antimicrobial activity of nigella sativa seed extract
title_full_unstemmed	Antimicrobial activity of nigella sativa seed extract
title_sort	antimicrobial activity of nigella sativa seed extract
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/5896/ http://journalarticle.ukm.my/5896/ http://journalarticle.ukm.my/5896/1/04%2520NorAishah.pdf
first_indexed	2023-09-18T19:45:28Z
last_indexed	2023-09-18T19:45:28Z
_version_	1777405869980385280

Antimicrobial activity of nigella sativa seed extract

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