Characterization of fusarium semitectum isolates from vegetable fruits

Fusarium semitectum is a widespread species occurring in various types of substrate. Fifteen F. semitectum isolates were recovered from several types of vegetable fruits showing fruit rot symptoms, namely long bean (Vigna sesquilpedalis), okra (Abelmoschus esculentus), loofa (Luffa acutangula), bitt...

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Main Authors:	Latiffah, Z., Nurul Huda, M.S., Tengku Ahmad Akram, T.M.A
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6156/ http://journalarticle.ukm.my/6156/ http://journalarticle.ukm.my/6156/1/10_Z._Latiffah.pdf

id	ukm-6156
recordtype	eprints
spelling	ukm-61562016-12-14T06:40:30Z http://journalarticle.ukm.my/6156/ Characterization of fusarium semitectum isolates from vegetable fruits Latiffah, Z. Nurul Huda, M.S. Tengku Ahmad Akram, T.M.A Fusarium semitectum is a widespread species occurring in various types of substrate. Fifteen F. semitectum isolates were recovered from several types of vegetable fruits showing fruit rot symptoms, namely long bean (Vigna sesquilpedalis), okra (Abelmoschus esculentus), loofa (Luffa acutangula), bitter gourd (Momordica charantia), cucumber (Cucumis sativus) and green chilli (Capsicum annum). The identification of the F. semitectum isolates was based on morphological characteristics of macroconidia and microconidia, presence of mesoconidia and colony pigmentation. The isolates were then characterized using vegetative compatibility group (VCG) and sequencing of translation elongation factor-1α gene (TEF-1α). From VCG analysis, 11 isolates were assigned to six VCGs and another four isolates were self-incompatible. The results of the phylogenetic analysis using TEF-1α sequences showed that the isolates were phyogenetically distinct although the morphological characteristics were very similar and generally the grouping of the isolates was not according to the host. Both VCG and phylogenetic analyses indicated that F. semitectum isolates from vegetable fruits were highly variable which suggested that the isolates belong to a species complex. Universiti Kebangsaan Malaysia 2013-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6156/1/10_Z._Latiffah.pdf Latiffah, Z. and Nurul Huda, M.S. and Tengku Ahmad Akram, T.M.A (2013) Characterization of fusarium semitectum isolates from vegetable fruits. Sains Malaysiana, 42 (5). pp. 629-633. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Fusarium semitectum is a widespread species occurring in various types of substrate. Fifteen F. semitectum isolates were recovered from several types of vegetable fruits showing fruit rot symptoms, namely long bean (Vigna sesquilpedalis), okra (Abelmoschus esculentus), loofa (Luffa acutangula), bitter gourd (Momordica charantia), cucumber (Cucumis sativus) and green chilli (Capsicum annum). The identification of the F. semitectum isolates was based on morphological characteristics of macroconidia and microconidia, presence of mesoconidia and colony pigmentation. The isolates were then characterized using vegetative compatibility group (VCG) and sequencing of translation elongation factor-1α gene (TEF-1α). From VCG analysis, 11 isolates were assigned to six VCGs and another four isolates were self-incompatible. The results of the phylogenetic analysis using TEF-1α sequences showed that the isolates were phyogenetically distinct although the morphological characteristics were very similar and generally the grouping of the isolates was not according to the host. Both VCG and phylogenetic analyses indicated that F. semitectum isolates from vegetable fruits were highly variable which suggested that the isolates belong to a species complex.
format	Article
author	Latiffah, Z. Nurul Huda, M.S. Tengku Ahmad Akram, T.M.A
spellingShingle	Latiffah, Z. Nurul Huda, M.S. Tengku Ahmad Akram, T.M.A Characterization of fusarium semitectum isolates from vegetable fruits
author_facet	Latiffah, Z. Nurul Huda, M.S. Tengku Ahmad Akram, T.M.A
author_sort	Latiffah, Z.
title	Characterization of fusarium semitectum isolates from vegetable fruits
title_short	Characterization of fusarium semitectum isolates from vegetable fruits
title_full	Characterization of fusarium semitectum isolates from vegetable fruits
title_fullStr	Characterization of fusarium semitectum isolates from vegetable fruits
title_full_unstemmed	Characterization of fusarium semitectum isolates from vegetable fruits
title_sort	characterization of fusarium semitectum isolates from vegetable fruits
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6156/ http://journalarticle.ukm.my/6156/ http://journalarticle.ukm.my/6156/1/10_Z._Latiffah.pdf
first_indexed	2023-09-18T19:46:08Z
last_indexed	2023-09-18T19:46:08Z
_version_	1777405912292524032

Characterization of fusarium semitectum isolates from vegetable fruits

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