Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)

The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 μg/kg,...

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Main Authors: Adebiyi, Fatimat Adenike, Siti Shapor Siraj, Sharr Azni Harmin, Christianus, Annie
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/6157/
http://journalarticle.ukm.my/6157/
http://journalarticle.ukm.my/6157/1/11_Fatimat.pdf
id ukm-6157
recordtype eprints
spelling ukm-61572016-12-14T06:40:30Z http://journalarticle.ukm.my/6157/ Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840) Adebiyi, Fatimat Adenike Siti Shapor Siraj, Sharr Azni Harmin, Christianus, Annie The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 μg/kg, 20 μg/kg and 50 μg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 μg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 μg/kg and 50 μg/kg BW GnRHa treatments and a dose of 5 μg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 μg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus. Universiti Kebangsaan Malaysia 2013-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6157/1/11_Fatimat.pdf Adebiyi, Fatimat Adenike and Siti Shapor Siraj, and Sharr Azni Harmin, and Christianus, Annie (2013) Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840). Sains Malaysiana, 42 (5). pp. 635-642. ISSN 0126-6039 http://www.ukm.my/jsm/
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institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 μg/kg, 20 μg/kg and 50 μg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 μg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 μg/kg and 50 μg/kg BW GnRHa treatments and a dose of 5 μg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 μg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus.
format Article
author Adebiyi, Fatimat Adenike
Siti Shapor Siraj,
Sharr Azni Harmin,
Christianus, Annie
spellingShingle Adebiyi, Fatimat Adenike
Siti Shapor Siraj,
Sharr Azni Harmin,
Christianus, Annie
Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
author_facet Adebiyi, Fatimat Adenike
Siti Shapor Siraj,
Sharr Azni Harmin,
Christianus, Annie
author_sort Adebiyi, Fatimat Adenike
title Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
title_short Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
title_full Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
title_fullStr Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
title_full_unstemmed Effects of GnRHa on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
title_sort effects of gnrha on plasma sex steroid hormones of river catfish hemibagrus nemurus(valenciennes 1840)
publisher Universiti Kebangsaan Malaysia
publishDate 2013
url http://journalarticle.ukm.my/6157/
http://journalarticle.ukm.my/6157/
http://journalarticle.ukm.my/6157/1/11_Fatimat.pdf
first_indexed 2023-09-18T19:46:08Z
last_indexed 2023-09-18T19:46:08Z
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