Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets

In forensic entomology practice, it is more common to use raw animal tissue to breed dipteran larvae and it often brings unpleasant odour in the laboratory. Few studies suggested the use of synthetic diets, mainly agar-based media, as alternatives to animal tissue but it is rarely being practiced in...

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Main Authors:	Raja Muhammad Zuha, Supriyani Mustamin, Balkhis Bashuri, Nazni Wasi Ahmad, Baharudin Omar
Format:	Article
Language:	English
Published:	penerbit ukm 2012
Subjects:	RA Public aspects of medicine
Online Access:	http://journalarticle.ukm.my/6368/ http://journalarticle.ukm.my/6368/1/49-52.pdf

id	ukm-6368
recordtype	eprints
spelling	ukm-63682016-12-14T06:40:59Z http://journalarticle.ukm.my/6368/ Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets Raja Muhammad Zuha, Supriyani Mustamin, Balkhis Bashuri, Nazni Wasi Ahmad, Baharudin Omar, RA Public aspects of medicine In forensic entomology practice, it is more common to use raw animal tissue to breed dipteran larvae and it often brings unpleasant odour in the laboratory. Few studies suggested the use of synthetic diets, mainly agar-based media, as alternatives to animal tissue but it is rarely being practiced in forensic entomology laboratory. The present study observed the growth of a forensically important fly, Megaselia scalaris (Loew) on raw cow’s liver, nutrient agar, casein agar and cow’s liver agar. A total of 100 M. scalaris eggs were transferred each into the different media and placed in an incubator at 30°C in a continuous dark condition. Data on length and developmental period were collected by randomly sampling three of the largest larvae from each rearing media, twice a day at 0900 and 1500 hours until pupariation. M. scalaris larvae reared on raw cow’s liver recorded the highest mean length (4.23 ± 1.96 mm) followed by cow’s liver agar (3.79 ± 1.62 mm), casein agar (3.14 ± 1.16 mm) and nutrient agar (3.09 ± 1.11 mm). Larval length in raw liver and liver agar were significantly different from those in nutrient and casein agar (p < 0.05). Larvae bred in liver agar and raw liver recorded the shortest larval duration before entering the post-feeding stage (89 hours), followed by nutrient agar (119 hours) and casein agar (184 hours). Total developmental time from oviposition until adult emergence for M. scalaris in liver agar and raw liver was approximately 163 hours. All puparia in nutrient agar and casein agar failed to hatch. This research highlighted the potential use of cow’s liver agar as an alternative diet of raw liver to culture M. scalaris in laboratory penerbit ukm 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6368/1/49-52.pdf Raja Muhammad Zuha, and Supriyani Mustamin, and Balkhis Bashuri, and Nazni Wasi Ahmad, and Baharudin Omar, (2012) Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets. Jurnal Sains Kesihatan Malaysia, 10 (2). pp. 49-52. ISSN 1675-8161
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
topic	RA Public aspects of medicine
spellingShingle	RA Public aspects of medicine Raja Muhammad Zuha, Supriyani Mustamin, Balkhis Bashuri, Nazni Wasi Ahmad, Baharudin Omar, Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
description	In forensic entomology practice, it is more common to use raw animal tissue to breed dipteran larvae and it often brings unpleasant odour in the laboratory. Few studies suggested the use of synthetic diets, mainly agar-based media, as alternatives to animal tissue but it is rarely being practiced in forensic entomology laboratory. The present study observed the growth of a forensically important fly, Megaselia scalaris (Loew) on raw cow’s liver, nutrient agar, casein agar and cow’s liver agar. A total of 100 M. scalaris eggs were transferred each into the different media and placed in an incubator at 30°C in a continuous dark condition. Data on length and developmental period were collected by randomly sampling three of the largest larvae from each rearing media, twice a day at 0900 and 1500 hours until pupariation. M. scalaris larvae reared on raw cow’s liver recorded the highest mean length (4.23 ± 1.96 mm) followed by cow’s liver agar (3.79 ± 1.62 mm), casein agar (3.14 ± 1.16 mm) and nutrient agar (3.09 ± 1.11 mm). Larval length in raw liver and liver agar were significantly different from those in nutrient and casein agar (p < 0.05). Larvae bred in liver agar and raw liver recorded the shortest larval duration before entering the post-feeding stage (89 hours), followed by nutrient agar (119 hours) and casein agar (184 hours). Total developmental time from oviposition until adult emergence for M. scalaris in liver agar and raw liver was approximately 163 hours. All puparia in nutrient agar and casein agar failed to hatch. This research highlighted the potential use of cow’s liver agar as an alternative diet of raw liver to culture M. scalaris in laboratory
format	Article
author	Raja Muhammad Zuha, Supriyani Mustamin, Balkhis Bashuri, Nazni Wasi Ahmad, Baharudin Omar,
author_facet	Raja Muhammad Zuha, Supriyani Mustamin, Balkhis Bashuri, Nazni Wasi Ahmad, Baharudin Omar,
author_sort	Raja Muhammad Zuha,
title	Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
title_short	Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
title_full	Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
title_fullStr	Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
title_full_unstemmed	Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets
title_sort	development of a forensically important fly, megaselia scalaris (loew) (diptera: phoridae) on cow’s liver and various agar-based diets
publisher	penerbit ukm
publishDate	2012
url	http://journalarticle.ukm.my/6368/ http://journalarticle.ukm.my/6368/1/49-52.pdf
first_indexed	2023-09-18T19:46:41Z
last_indexed	2023-09-18T19:46:41Z
_version_	1777405947368439808

Development of a forensically important fly, Megaselia scalaris (Loew) (Diptera: Phoridae) on cow’s Liver and various agar-based diets

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