In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)

Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, dev...

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Main Authors:	Samanthi, P.W., Mohd Puad, A., Suhaimi, N., Kumar, S.M., Nor Aini, A.S.
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6554/ http://journalarticle.ukm.my/6554/ http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf

id	ukm-6554
recordtype	eprints
spelling	ukm-65542016-12-14T06:41:31Z http://journalarticle.ukm.my/6554/ In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.) Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, development of plant regeneration through callus is important for in vitro genetic manipulation of kenaf. Currently development of successful genetic transformation of kenaf is through in planta transformation means. In vitro shoot regeneration was conducted using leaf explants from varieties V36 and G4 treated to three different combinations of N6 Benzyl adenine (BA) and Indole-3-butyric acid (IBA). High percentage of healthy callus induction was produced in MS medium supplemented with combination of 1.5 mgL-1 BA and 0.5 mgL-1 IBA. In addition 68.7% plant regeneration was obtained in MS medium supplemented with 0.3 mgL-1 GA3. All plantlets produced roots in hormone free medium. There was no significant difference among varieties in terms of callus induction (number of callus) and plant regeneration (number of plantlets). This protocol is useful to be used for the development of gene transformation protocol of kenaf through callus. Universiti Kebangsaan Malaysia 2013-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf Samanthi, P.W. and Mohd Puad, A. and Suhaimi, N. and Kumar, S.M. and Nor Aini, A.S. (2013) In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.). Sains Malaysiana, 42 (10). pp. 1505-1510. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Kenaf (Hibiscus cannabinus L.) is a versatile plant with multiuse ranging from animal feed to a wide variety of biocomposite products such as pulp and paper and fibre reinforce plastic. Therefore genetically improved planting materials are needed to tailor made requirement of the industry. Thus, development of plant regeneration through callus is important for in vitro genetic manipulation of kenaf. Currently development of successful genetic transformation of kenaf is through in planta transformation means. In vitro shoot regeneration was conducted using leaf explants from varieties V36 and G4 treated to three different combinations of N6 Benzyl adenine (BA) and Indole-3-butyric acid (IBA). High percentage of healthy callus induction was produced in MS medium supplemented with combination of 1.5 mgL-1 BA and 0.5 mgL-1 IBA. In addition 68.7% plant regeneration was obtained in MS medium supplemented with 0.3 mgL-1 GA3. All plantlets produced roots in hormone free medium. There was no significant difference among varieties in terms of callus induction (number of callus) and plant regeneration (number of plantlets). This protocol is useful to be used for the development of gene transformation protocol of kenaf through callus.
format	Article
author	Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S.
spellingShingle	Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S. In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
author_facet	Samanthi, P.W. Mohd Puad, A. Suhaimi, N. Kumar, S.M. Nor Aini, A.S.
author_sort	Samanthi, P.W.
title	In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
title_short	In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
title_full	In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
title_fullStr	In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
title_full_unstemmed	In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)
title_sort	in vitro shoot regeneration from leaf explants of kenaf (hibiscus cannabinus l.)
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6554/ http://journalarticle.ukm.my/6554/ http://journalarticle.ukm.my/6554/1/21_P.W._Samanthi.pdf
first_indexed	2023-09-18T19:47:15Z
last_indexed	2023-09-18T19:47:15Z
_version_	1777405982511464448

In vitro shoot regeneration from leaf explants of kenaf (Hibiscus cannabinus L.)

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