Electron microscopy studies of the effects of garlic extract against trichophyton rubrum

Trichophyton rubrum is one of dermatophytes that penetrates keratinized tissues such as skin, hair and nail of human and animals. Recently, antifungal drugs such as imodazole and triazole was found to cause side effects, toxicity to patients and also not very efficient due to resistance to these dru...

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Main Authors:	Farzad Aala, Umi Kalsom Yusuf, Rosimah Nulit
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6622/ http://journalarticle.ukm.my/6622/ http://journalarticle.ukm.my/6622/1/07_Farzad_Aala-1.pdf

id	ukm-6622
recordtype	eprints
spelling	ukm-66222016-12-14T06:41:43Z http://journalarticle.ukm.my/6622/ Electron microscopy studies of the effects of garlic extract against trichophyton rubrum Farzad Aala, Umi Kalsom Yusuf, Rosimah Nulit, Trichophyton rubrum is one of dermatophytes that penetrates keratinized tissues such as skin, hair and nail of human and animals. Recently, antifungal drugs such as imodazole and triazole was found to cause side effects, toxicity to patients and also not very efficient due to resistance to these drugs. As an alternative, some plants extract had been used to treat dermatophytes. This studies was done using Garlic extract (Allium sativum) to evaluate its effects on the growth of hypha of Trichophyton using Electron miscroscopy. Garlic had been known to posses antimicrobial, antiinflammatory, antithrombotic and antitumor activities. This studies found that garlic extract as low as 4 mg/mL inhibit the growth of hypha. Scanning electron microscopy studies revealed that hypha treated with garlic extract showed shrinkage, flat and cell wall demolition, similar to hypha treated with allicin (positive control) having rough surface, shrinkage and distortion. The tip of hypa became large after treatment with garlic extract. Transmission electron microscopy studies also found that hypha treated with allicin display cell wall thickening, local thickening, destruction of cytoplasmic content, mean while hypha treated with garlic extract exhibited cell wall thickening, disordered hyphal tip and desolution of cytoplasmic compartments and similar with hypha treated with allicin. These results showed that garlic extract and pure allicin could be use as an alternative to treat dermatophytes. Universiti Kebangsaan Malaysia 2013-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6622/1/07_Farzad_Aala-1.pdf Farzad Aala, and Umi Kalsom Yusuf, and Rosimah Nulit, (2013) Electron microscopy studies of the effects of garlic extract against trichophyton rubrum. Sains Malaysiana, 42 (11). pp. 1585-1590. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Trichophyton rubrum is one of dermatophytes that penetrates keratinized tissues such as skin, hair and nail of human and animals. Recently, antifungal drugs such as imodazole and triazole was found to cause side effects, toxicity to patients and also not very efficient due to resistance to these drugs. As an alternative, some plants extract had been used to treat dermatophytes. This studies was done using Garlic extract (Allium sativum) to evaluate its effects on the growth of hypha of Trichophyton using Electron miscroscopy. Garlic had been known to posses antimicrobial, antiinflammatory, antithrombotic and antitumor activities. This studies found that garlic extract as low as 4 mg/mL inhibit the growth of hypha. Scanning electron microscopy studies revealed that hypha treated with garlic extract showed shrinkage, flat and cell wall demolition, similar to hypha treated with allicin (positive control) having rough surface, shrinkage and distortion. The tip of hypa became large after treatment with garlic extract. Transmission electron microscopy studies also found that hypha treated with allicin display cell wall thickening, local thickening, destruction of cytoplasmic content, mean while hypha treated with garlic extract exhibited cell wall thickening, disordered hyphal tip and desolution of cytoplasmic compartments and similar with hypha treated with allicin. These results showed that garlic extract and pure allicin could be use as an alternative to treat dermatophytes.
format	Article
author	Farzad Aala, Umi Kalsom Yusuf, Rosimah Nulit,
spellingShingle	Farzad Aala, Umi Kalsom Yusuf, Rosimah Nulit, Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
author_facet	Farzad Aala, Umi Kalsom Yusuf, Rosimah Nulit,
author_sort	Farzad Aala,
title	Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
title_short	Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
title_full	Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
title_fullStr	Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
title_full_unstemmed	Electron microscopy studies of the effects of garlic extract against trichophyton rubrum
title_sort	electron microscopy studies of the effects of garlic extract against trichophyton rubrum
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6622/ http://journalarticle.ukm.my/6622/ http://journalarticle.ukm.my/6622/1/07_Farzad_Aala-1.pdf
first_indexed	2023-09-18T19:47:27Z
last_indexed	2023-09-18T19:47:27Z
_version_	1777405994859495424

Electron microscopy studies of the effects of garlic extract against trichophyton rubrum

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