Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.

The potential of Centella asiatica (CA) as an antioxidant and anti-inflammatory agent has been well described. However the extraction method which gives the best yield is debatable. In this study, we evaluated three different methods of extractions and compared the extracts in terms of antioxidant,...

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Main Authors: Nurlaily A, Noor Baitee AR, Musalmah M
Format: Article
Language:English
Published: Penerbit UKM 2012
Online Access:http://journalarticle.ukm.my/6738/
http://journalarticle.ukm.my/6738/
http://journalarticle.ukm.my/6738/1/01-MS151_%2862-72%29.pdf
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spelling ukm-67382016-12-14T06:42:04Z http://journalarticle.ukm.my/6738/ Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside. Nurlaily A, Noor Baitee AR, Musalmah M, The potential of Centella asiatica (CA) as an antioxidant and anti-inflammatory agent has been well described. However the extraction method which gives the best yield is debatable. In this study, we evaluated three different methods of extractions and compared the extracts in terms of antioxidant, anti-inflammatory activities as well as the contents of its bioactive compounds, asiaticoside and madecassoside. Centella asiatica was extracted using ethanol, methanol and aqueous extraction methods. The extracts were then measured for their phenolic contents using Folin-Ciocalteu reagent. Asiaticoside and madecassoside were determined using HPLC. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DHPP) and ferric reducing antioxidant power (FRAP) assays. Anti-inflammatory activities were determined by the ability of the extracts to inhibit the inflammatory pathway enzyme, COX-1 and COX-2 as well as their ability to protect fibroblasts against 12-O-tetradecanoylphorbol-13-acetate (TPA) -induced production of prostaglandin E2 (PGE2). Results showed that the level of phenolic constituents, asiaticoside and madecassoside were highest in the ethanol, followed by methanol and then aqueous extracts (17.76 g/100g, 15.52 g/100g, 13.16 g/100g for phenolics, 42.86 mg/g, 36.37 mg/g, 2.82 mg/g for asiaticoside and 18.66 mg/g, 15.87 mg/g, 3.75 mg/g for madecassoside respectively. All extracts showed considerable antioxidant activity compared to the positive controls. The extracts, asiaticosside and madecassoside inhibited both COX-1 and COX-2 and suppressed the TPA-induced production of PGE2. The ethanol and methanol extracts were stronger COX inhibitors and more potent suppressor of PGE2 formation than aqueous extract. Thus although the aqueous extract showed higher antioxidant potential, in terms of anti-inflammatory activities, the hydrophobic solvents, ethanol and methanol, proved to be the better extraction method for Centella asiatica. Penerbit UKM 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6738/1/01-MS151_%2862-72%29.pdf Nurlaily A, and Noor Baitee AR, and Musalmah M, (2012) Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside. Medicine & Health, 7 (2). pp. 62-72. ISSN 1823-2140 http://www.medicineandhealthukm.com/
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description The potential of Centella asiatica (CA) as an antioxidant and anti-inflammatory agent has been well described. However the extraction method which gives the best yield is debatable. In this study, we evaluated three different methods of extractions and compared the extracts in terms of antioxidant, anti-inflammatory activities as well as the contents of its bioactive compounds, asiaticoside and madecassoside. Centella asiatica was extracted using ethanol, methanol and aqueous extraction methods. The extracts were then measured for their phenolic contents using Folin-Ciocalteu reagent. Asiaticoside and madecassoside were determined using HPLC. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DHPP) and ferric reducing antioxidant power (FRAP) assays. Anti-inflammatory activities were determined by the ability of the extracts to inhibit the inflammatory pathway enzyme, COX-1 and COX-2 as well as their ability to protect fibroblasts against 12-O-tetradecanoylphorbol-13-acetate (TPA) -induced production of prostaglandin E2 (PGE2). Results showed that the level of phenolic constituents, asiaticoside and madecassoside were highest in the ethanol, followed by methanol and then aqueous extracts (17.76 g/100g, 15.52 g/100g, 13.16 g/100g for phenolics, 42.86 mg/g, 36.37 mg/g, 2.82 mg/g for asiaticoside and 18.66 mg/g, 15.87 mg/g, 3.75 mg/g for madecassoside respectively. All extracts showed considerable antioxidant activity compared to the positive controls. The extracts, asiaticosside and madecassoside inhibited both COX-1 and COX-2 and suppressed the TPA-induced production of PGE2. The ethanol and methanol extracts were stronger COX inhibitors and more potent suppressor of PGE2 formation than aqueous extract. Thus although the aqueous extract showed higher antioxidant potential, in terms of anti-inflammatory activities, the hydrophobic solvents, ethanol and methanol, proved to be the better extraction method for Centella asiatica.
format Article
author Nurlaily A,
Noor Baitee AR,
Musalmah M,
spellingShingle Nurlaily A,
Noor Baitee AR,
Musalmah M,
Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
author_facet Nurlaily A,
Noor Baitee AR,
Musalmah M,
author_sort Nurlaily A,
title Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
title_short Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
title_full Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
title_fullStr Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
title_full_unstemmed Comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (L.) urban and its active compounds, asiaticoside and madecassoside.
title_sort comparative antioxidant and anti-inflammatory activity of different extracts of centella asiatica (l.) urban and its active compounds, asiaticoside and madecassoside.
publisher Penerbit UKM
publishDate 2012
url http://journalarticle.ukm.my/6738/
http://journalarticle.ukm.my/6738/
http://journalarticle.ukm.my/6738/1/01-MS151_%2862-72%29.pdf
first_indexed 2023-09-18T19:47:45Z
last_indexed 2023-09-18T19:47:45Z
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