Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus

Tiger’s Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolys...

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Main Authors:	Wei, Hong Lai, Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/6817/ http://journalarticle.ukm.my/6817/ http://journalarticle.ukm.my/6817/1/09_Wei_Hong_Lai.pdf

id	ukm-6817
recordtype	eprints
spelling	ukm-68172016-12-14T06:42:17Z http://journalarticle.ukm.my/6817/ Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus Wei, Hong Lai Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh, Tiger’s Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium’s composition. Universiti Kebangsaan Malaysia 2014-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6817/1/09_Wei_Hong_Lai.pdf Wei, Hong Lai and Saadiah Mohd Salleh, and Fauzi Daud, and Zamri Zainal, and Abas Mazni Othman, and Norihan Mohd Saleh, (2014) Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus. Sains Malaysiana, 43 (1). pp. 73-80. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Tiger’s Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium’s composition.
format	Article
author	Wei, Hong Lai Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh,
spellingShingle	Wei, Hong Lai Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh, Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
author_facet	Wei, Hong Lai Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh,
author_sort	Wei, Hong Lai
title	Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
title_short	Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
title_full	Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
title_fullStr	Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
title_full_unstemmed	Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
title_sort	optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/6817/ http://journalarticle.ukm.my/6817/ http://journalarticle.ukm.my/6817/1/09_Wei_Hong_Lai.pdf
first_indexed	2023-09-18T19:47:58Z
last_indexed	2023-09-18T19:47:58Z
_version_	1777406028103548928

Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus

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