Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making

Fermentation of legumes is a well-recognised preserving method that not only diversifies the sensory attributes of the legume-based foods but also enhances its nutritional value. This study aimed to evaluate the changes in amino acids content of soybean, garbanzo bean and groundnut upon pre-treatmen...

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Main Authors: Aishah Bujang, Nurul Akmal Taib
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/7049/
http://journalarticle.ukm.my/7049/
http://journalarticle.ukm.my/7049/1/08_Aishah_Bujang.pdf
id ukm-7049
recordtype eprints
spelling ukm-70492016-12-14T06:42:57Z http://journalarticle.ukm.my/7049/ Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making Aishah Bujang, Nurul Akmal Taib, Fermentation of legumes is a well-recognised preserving method that not only diversifies the sensory attributes of the legume-based foods but also enhances its nutritional value. This study aimed to evaluate the changes in amino acids content of soybean, garbanzo bean and groundnut upon pre-treatments (soaking and steaming) and fermentation times (18, 24 and 30 h). The amino acids analysis was performed with a HPLC system using AccQTag method. The results obtained showed that raw soybean contain the highest amount of essential and non-essential amino acids followed by raw garbanzo bean and groundnut. After the soaking process, the amount of amino acids obtained was significantly increased in all three legumes. However, the steaming process was found to cause significant decrease in the amino acids content. After the steaming process, the legumes were inoculated with Rhizopus oligosporus and incubated at 30oC and 65% RH. At 18 h of fermentation time, the amount of total amino acids were found to increase by 26% in fermented groundnut and 16% in both soybean and garbanzo bean compared to after steaming. Further fermentation time of 24 h shows further enhancement in the amino acids content in all three legumes. Groundnut shows the highest increased of 71% in total amino acids content followed by 63% increased in garbanzo bean and 53% increase in soybean. However, prolong fermentation time of 30 h shows a decrease in total amino acids content in all three legumes which indicates the slow process of mould degradation have occurred. Therefore, fermented legumes must be consumed at the appropriate time of fermentation in order to get the benefit from the highest accumulation of amino acids content. Universiti Kebangsaan Malaysia 2014-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7049/1/08_Aishah_Bujang.pdf Aishah Bujang, and Nurul Akmal Taib, (2014) Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making. Sains Malaysiana, 43 (4). pp. 551-557. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Fermentation of legumes is a well-recognised preserving method that not only diversifies the sensory attributes of the legume-based foods but also enhances its nutritional value. This study aimed to evaluate the changes in amino acids content of soybean, garbanzo bean and groundnut upon pre-treatments (soaking and steaming) and fermentation times (18, 24 and 30 h). The amino acids analysis was performed with a HPLC system using AccQTag method. The results obtained showed that raw soybean contain the highest amount of essential and non-essential amino acids followed by raw garbanzo bean and groundnut. After the soaking process, the amount of amino acids obtained was significantly increased in all three legumes. However, the steaming process was found to cause significant decrease in the amino acids content. After the steaming process, the legumes were inoculated with Rhizopus oligosporus and incubated at 30oC and 65% RH. At 18 h of fermentation time, the amount of total amino acids were found to increase by 26% in fermented groundnut and 16% in both soybean and garbanzo bean compared to after steaming. Further fermentation time of 24 h shows further enhancement in the amino acids content in all three legumes. Groundnut shows the highest increased of 71% in total amino acids content followed by 63% increased in garbanzo bean and 53% increase in soybean. However, prolong fermentation time of 30 h shows a decrease in total amino acids content in all three legumes which indicates the slow process of mould degradation have occurred. Therefore, fermented legumes must be consumed at the appropriate time of fermentation in order to get the benefit from the highest accumulation of amino acids content.
format Article
author Aishah Bujang,
Nurul Akmal Taib,
spellingShingle Aishah Bujang,
Nurul Akmal Taib,
Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
author_facet Aishah Bujang,
Nurul Akmal Taib,
author_sort Aishah Bujang,
title Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
title_short Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
title_full Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
title_fullStr Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
title_full_unstemmed Changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
title_sort changes on amino acids content in soybean, garbanzo bean and groundnut during pre-treatments and tempe making
publisher Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/7049/
http://journalarticle.ukm.my/7049/
http://journalarticle.ukm.my/7049/1/08_Aishah_Bujang.pdf
first_indexed 2023-09-18T19:48:37Z
last_indexed 2023-09-18T19:48:37Z
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