The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots

Currently, bio-pesticide is relatively harmless to human and environment and thus desirable for its use in the control of insect vectors. Bio-pesticide has been increasingly important in both scale commercial agriculture and small plot, subsistence farming. One of the sources for bio-pesticide is ‘t...

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Main Authors:	Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7151/ http://journalarticle.ukm.my/7151/ http://journalarticle.ukm.my/7151/1/08_Saiful_Irwan.pdf

id	ukm-7151
recordtype	eprints
spelling	ukm-71512016-12-14T06:43:14Z http://journalarticle.ukm.my/7151/ The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz, Currently, bio-pesticide is relatively harmless to human and environment and thus desirable for its use in the control of insect vectors. Bio-pesticide has been increasingly important in both scale commercial agriculture and small plot, subsistence farming. One of the sources for bio-pesticide is ‘tuba’ plant, known as Derris elliptica. Derris elliptica contains bio-active compounds known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study was carried out to investigate the appropriate processing parameters with the aimed to acquire high yield of rotenone (mg) and concentration (mg/mL) of the exhaustive extraction process by evaluating the kinetics of the normal soaking extraction (NSE) method. The raw plants were collected from Kota Johor Lama, Johor and sorted to collect the root. The roots were sorted into 2 types of raw material particles size (mm in diameter) prior to the extraction process. Rotenone from the root part was extracted by using a NSE method at ambient temperature of 27±1oC. Three types of solvents and 2 solvent-to-solid ratios were utilized throughout the extraction process. The extraction was carried out for 50 h and the fractions of the liquid crude extract were collected for each interval time (2 h/mL/fraction) and further cleaned up to remove any fine debris prior to the determination of rotenone content (mg) and its concentration (mg/mL) via reverse-phase high performance liquid chromatography (RP-HPLC). From the kinetics result obtained, it was found that the fine Derris elliptica roots with particles size of 0.5 - 2 mm in diameter and solvent-to-solid ratio of 10 mL/g of acetone solvent system were considered the best processing parameters to procure high yield of rotenone and its concentration. Universiti Kebangsaan Malaysia 2014-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7151/1/08_Saiful_Irwan.pdf Saiful Irwan Zubairi, and Mohamad Roji Sarmidi, and Ramlan Abdul Aziz, (2014) The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots. Sains Malaysiana, 43 (5). pp. 707-713. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Currently, bio-pesticide is relatively harmless to human and environment and thus desirable for its use in the control of insect vectors. Bio-pesticide has been increasingly important in both scale commercial agriculture and small plot, subsistence farming. One of the sources for bio-pesticide is ‘tuba’ plant, known as Derris elliptica. Derris elliptica contains bio-active compounds known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study was carried out to investigate the appropriate processing parameters with the aimed to acquire high yield of rotenone (mg) and concentration (mg/mL) of the exhaustive extraction process by evaluating the kinetics of the normal soaking extraction (NSE) method. The raw plants were collected from Kota Johor Lama, Johor and sorted to collect the root. The roots were sorted into 2 types of raw material particles size (mm in diameter) prior to the extraction process. Rotenone from the root part was extracted by using a NSE method at ambient temperature of 27±1oC. Three types of solvents and 2 solvent-to-solid ratios were utilized throughout the extraction process. The extraction was carried out for 50 h and the fractions of the liquid crude extract were collected for each interval time (2 h/mL/fraction) and further cleaned up to remove any fine debris prior to the determination of rotenone content (mg) and its concentration (mg/mL) via reverse-phase high performance liquid chromatography (RP-HPLC). From the kinetics result obtained, it was found that the fine Derris elliptica roots with particles size of 0.5 - 2 mm in diameter and solvent-to-solid ratio of 10 mL/g of acetone solvent system were considered the best processing parameters to procure high yield of rotenone and its concentration.
format	Article
author	Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz,
spellingShingle	Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz, The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
author_facet	Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz,
author_sort	Saiful Irwan Zubairi,
title	The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
title_short	The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
title_full	The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
title_fullStr	The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
title_full_unstemmed	The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
title_sort	effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7151/ http://journalarticle.ukm.my/7151/ http://journalarticle.ukm.my/7151/1/08_Saiful_Irwan.pdf
first_indexed	2023-09-18T19:48:54Z
last_indexed	2023-09-18T19:48:54Z
_version_	1777406086016401408

The effects of raw material particles size, types of solvents and solvent-to-solid ratio on the yield of rotenone extracted from derris elliptica roots

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