Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)

Numerous studies have reported on regeneration from apple somatic tissues, andorganogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, o...

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Main Authors:	Noormohammadi, Z., Farahani, F., Safarzadeh, M.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/7202/ http://journalarticle.ukm.my/7202/ http://journalarticle.ukm.my/7202/1/42_1_03.pdf

id	ukm-7202
recordtype	eprints
spelling	ukm-72022016-12-14T06:43:23Z http://journalarticle.ukm.my/7202/ Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106) Noormohammadi, Z. Farahani, F. Safarzadeh, M. Numerous studies have reported on regeneration from apple somatic tissues, andorganogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors during the regeneration process ismicro and macro elements in media culture and the type and concentration of cytokinin applied. For optimization of the elongation shoot and proliferation medium, two different macro and micro element formulations were tested: MS and DKW. The 2-isopentnyl adenine (2-ip) and benzyl adenine (BA) are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. The organogenic ability of explants can also be increased by a properly selected cytokinin treatment. Phytohormones (2 mgl-1 BA and 2 mgl-1 2-ip with 0.1 mgl-1 IBA) established for the proliferation of M26 and MM106 apple rootstocks. The both of rootstocks, the MS medium with BA (2 mgl-1) yielded in general the best shoot growth and elongation of branch also, DKW with 2-ip (2 mgl-1) showed the best callus formation and branching. Significantly better growth with the MS medium was also favored by BA as the cytokinin. Penerbit Universiti Kebangsaan Malaysia 2013-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7202/1/42_1_03.pdf Noormohammadi, Z. and Farahani, F. and Safarzadeh, M. (2013) Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106). Malaysian Applied Biology, 42 (1). pp. 25-33. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Numerous studies have reported on regeneration from apple somatic tissues, andorganogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors during the regeneration process ismicro and macro elements in media culture and the type and concentration of cytokinin applied. For optimization of the elongation shoot and proliferation medium, two different macro and micro element formulations were tested: MS and DKW. The 2-isopentnyl adenine (2-ip) and benzyl adenine (BA) are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. The organogenic ability of explants can also be increased by a properly selected cytokinin treatment. Phytohormones (2 mgl-1 BA and 2 mgl-1 2-ip with 0.1 mgl-1 IBA) established for the proliferation of M26 and MM106 apple rootstocks. The both of rootstocks, the MS medium with BA (2 mgl-1) yielded in general the best shoot growth and elongation of branch also, DKW with 2-ip (2 mgl-1) showed the best callus formation and branching. Significantly better growth with the MS medium was also favored by BA as the cytokinin.
format	Article
author	Noormohammadi, Z. Farahani, F. Safarzadeh, M.
spellingShingle	Noormohammadi, Z. Farahani, F. Safarzadeh, M. Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
author_facet	Noormohammadi, Z. Farahani, F. Safarzadeh, M.
author_sort	Noormohammadi, Z.
title	Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
title_short	Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
title_full	Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
title_fullStr	Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
title_full_unstemmed	Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)
title_sort	study of morphological traits changes in different media culture of two apple rootstocks (m26 and mm106)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/7202/ http://journalarticle.ukm.my/7202/ http://journalarticle.ukm.my/7202/1/42_1_03.pdf
first_indexed	2023-09-18T19:49:03Z
last_indexed	2023-09-18T19:49:03Z
_version_	1777406095758721024

Study of morphological traits changes in different media culture of two apple rootstocks (M26 and MM106)

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