Transposable elements and the detection of somaclonal variation in plant tissue culture

Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. Howev...

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Main Authors:	Azman, A.S., Mhiri, C., Grandbastien, M.A., Tam, S.M.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7283/ http://journalarticle.ukm.my/7283/ http://journalarticle.ukm.my/7283/1/43_1_01.pdf

id	ukm-7283
recordtype	eprints
spelling	ukm-72832016-12-14T06:43:37Z http://journalarticle.ukm.my/7283/ Transposable elements and the detection of somaclonal variation in plant tissue culture Azman, A.S. Mhiri, C., Grandbastien, M.A., Tam, S.M., Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. However, somaclonal variation has been observed in plant tissue culture due to either point mutations, transposition activity of mobile genetic elements, chromosomal rearrangements, or ploidy level changes, causing genetic instability. Tissue culture-induced mutations associated with transposable element activities have been reported from many plant studies, related to stress conditions during tissue culture such as wounding, exposure to hormones and/or specific compounds in the growth media, and genomic shocks from cytological changes. Transposable elements are repetitive DNA fragments with the ability to transpose from one region to another within a genome. Mutations that may occur during plant tissue culture suggest that the plantlets have to be regularly monitored. Methyl-sensitive transposon display (MSTD) is an advanced, efficient DNA fingerprinting technique that can simultaneously detect genetic variation, changes in transposable element insertion sites and the status of cytosine methylation of DNA in plant genomes. The main principles and notes for application of MSTD such as design of element-specific primers, identification of transposable element sequences in plant genomes, selection of the isochizomer enzymes used, and different classes of banding pattern shown are briefly discussed based on our preliminary work with Nicotiana benthamiana (Tnt1 retroelement) and Musa acuminata (Copia-33 Mad-I retroelement) examples. Penerbit Universiti Kebangsaan Malaysia 2014-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7283/1/43_1_01.pdf Azman, A.S. and Mhiri, C., and Grandbastien, M.A., and Tam, S.M., (2014) Transposable elements and the detection of somaclonal variation in plant tissue culture. Malaysian Applied Biology, 43 (1). pp. 1-12. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. However, somaclonal variation has been observed in plant tissue culture due to either point mutations, transposition activity of mobile genetic elements, chromosomal rearrangements, or ploidy level changes, causing genetic instability. Tissue culture-induced mutations associated with transposable element activities have been reported from many plant studies, related to stress conditions during tissue culture such as wounding, exposure to hormones and/or specific compounds in the growth media, and genomic shocks from cytological changes. Transposable elements are repetitive DNA fragments with the ability to transpose from one region to another within a genome. Mutations that may occur during plant tissue culture suggest that the plantlets have to be regularly monitored. Methyl-sensitive transposon display (MSTD) is an advanced, efficient DNA fingerprinting technique that can simultaneously detect genetic variation, changes in transposable element insertion sites and the status of cytosine methylation of DNA in plant genomes. The main principles and notes for application of MSTD such as design of element-specific primers, identification of transposable element sequences in plant genomes, selection of the isochizomer enzymes used, and different classes of banding pattern shown are briefly discussed based on our preliminary work with Nicotiana benthamiana (Tnt1 retroelement) and Musa acuminata (Copia-33 Mad-I retroelement) examples.
format	Article
author	Azman, A.S. Mhiri, C., Grandbastien, M.A., Tam, S.M.,
spellingShingle	Azman, A.S. Mhiri, C., Grandbastien, M.A., Tam, S.M., Transposable elements and the detection of somaclonal variation in plant tissue culture
author_facet	Azman, A.S. Mhiri, C., Grandbastien, M.A., Tam, S.M.,
author_sort	Azman, A.S.
title	Transposable elements and the detection of somaclonal variation in plant tissue culture
title_short	Transposable elements and the detection of somaclonal variation in plant tissue culture
title_full	Transposable elements and the detection of somaclonal variation in plant tissue culture
title_fullStr	Transposable elements and the detection of somaclonal variation in plant tissue culture
title_full_unstemmed	Transposable elements and the detection of somaclonal variation in plant tissue culture
title_sort	transposable elements and the detection of somaclonal variation in plant tissue culture
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7283/ http://journalarticle.ukm.my/7283/ http://journalarticle.ukm.my/7283/1/43_1_01.pdf
first_indexed	2023-09-18T19:49:17Z
last_indexed	2023-09-18T19:49:17Z
_version_	1777406110372724736

Transposable elements and the detection of somaclonal variation in plant tissue culture

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