Epitope mapping of Anti-YB-1 antibodies using truncated proteins

Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies,...

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Main Authors:	Wei, T.C., Shamsuddin, S.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7285/ http://journalarticle.ukm.my/7285/ http://journalarticle.ukm.my/7285/1/43_1_03.pdf

id	ukm-7285
recordtype	eprints
spelling	ukm-72852016-12-14T06:43:38Z http://journalarticle.ukm.my/7285/ Epitope mapping of Anti-YB-1 antibodies using truncated proteins Wei, T.C. Shamsuddin, S. Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies, three YB-1 truncated proteins were generated using bacterial expression system and their aberrant migration were reported. Epitope mapping analyses revealed anti-YB-1 A-16 antibody recognized epitope at 1-127 amino acid residues of YB-1, whereas 59-Q antibody was reactive with epitope at 61-127 amino acid residues. Based on our findings, 59-Q antibody was suggested used in the future study to avoid ambiguity. Penerbit Universiti Kebangsaan Malaysia 2014-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7285/1/43_1_03.pdf Wei, T.C. and Shamsuddin, S. (2014) Epitope mapping of Anti-YB-1 antibodies using truncated proteins. Malaysian Applied Biology, 43 (1). pp. 21-30. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Increased expression and nuclear localization of Y-box-binding protein-1 (YB-1) are associated with drug resistance and tumor progression. Detection of YB-1 primarily depends on the recognition of YB-1 antibody on its epitopes. To determine the epitopes binding of anti-YB-1 A-16 and 59-Q antibodies, three YB-1 truncated proteins were generated using bacterial expression system and their aberrant migration were reported. Epitope mapping analyses revealed anti-YB-1 A-16 antibody recognized epitope at 1-127 amino acid residues of YB-1, whereas 59-Q antibody was reactive with epitope at 61-127 amino acid residues. Based on our findings, 59-Q antibody was suggested used in the future study to avoid ambiguity.
format	Article
author	Wei, T.C. Shamsuddin, S.
spellingShingle	Wei, T.C. Shamsuddin, S. Epitope mapping of Anti-YB-1 antibodies using truncated proteins
author_facet	Wei, T.C. Shamsuddin, S.
author_sort	Wei, T.C.
title	Epitope mapping of Anti-YB-1 antibodies using truncated proteins
title_short	Epitope mapping of Anti-YB-1 antibodies using truncated proteins
title_full	Epitope mapping of Anti-YB-1 antibodies using truncated proteins
title_fullStr	Epitope mapping of Anti-YB-1 antibodies using truncated proteins
title_full_unstemmed	Epitope mapping of Anti-YB-1 antibodies using truncated proteins
title_sort	epitope mapping of anti-yb-1 antibodies using truncated proteins
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7285/ http://journalarticle.ukm.my/7285/ http://journalarticle.ukm.my/7285/1/43_1_03.pdf
first_indexed	2023-09-18T19:49:17Z
last_indexed	2023-09-18T19:49:17Z
_version_	1777406110671568896

Epitope mapping of Anti-YB-1 antibodies using truncated proteins

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