Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
Genetic variability of barley (Hordeum spp) considered very important, reducing their genetic diversity affects negatively their tolerance to stress factors, causing the great loose of yield and implies the risk of their genetic vulnerability. Thus, the knowledge of genetic diversity of barly germ...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/1/43_1_12.pdf |
| Summary: | Genetic variability of barley (Hordeum spp) considered very important, reducing their genetic diversity affects negatively
their tolerance to stress factors, causing the great loose of yield and implies the risk of their genetic vulnerability. Thus, the
knowledge of genetic diversity of barly germplasm is important for breeding programmes and their conservation. Random
amplified polymorphic DNA (RAPD) and SDS-PAGE techniques were used to examine the genetic variation among barley
varieties. To maximize the genome coverage nine barley varieties from diverse origins were selected and analyzed with 18
selected RAPD markers. A total of 116 amplified bands and 63 polymorphic allelic variants were detected, ranging from 3 to
6 per locus with an average of 3.937. Genetic distance calculated from RAPD data ranged from 0.357 to 0.767. The dendrogram
based on genetic distance estimation and PCA was produced two groupings that generally consistent with available genetic
information. Zanbaka and Tadmor/roh6 were found to be closely related. Their genetic relationship was also confirmed by
the facts that they shared a common ancestor and they were clustered together. A total of 14 unique bands (8 positives and 6
negatives) were identified out of 116 bands which could be considered for marker assisted selection. A slight range of
variation in SDS-protein banding patterns was observed. A total number of 12 bands with different molecular weights were
observed in SDS-PAGE, among these, only 5 bands were polymorphic. The dendrogram tree and PCA analysis showed that
Elkheir and Canela were genetically distanced from the other genotypes. According to the results of RAPD and SDS-PAGE,
a wide range of genetic diversity was observed among all varieties, indicating them as prime candidates of selective breeding
for specific traits and broadening the genetic base. |
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