Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)

Genetic variability of barley (Hordeum spp) considered very important, reducing their genetic diversity affects negatively their tolerance to stress factors, causing the great loose of yield and implies the risk of their genetic vulnerability. Thus, the knowledge of genetic diversity of barly germ...

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Main Author:	N.A., Tahir
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/1/43_1_12.pdf

id	ukm-7295
recordtype	eprints
spelling	ukm-72952016-12-14T06:43:40Z http://journalarticle.ukm.my/7295/ Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.) N.A., Tahir Genetic variability of barley (Hordeum spp) considered very important, reducing their genetic diversity affects negatively their tolerance to stress factors, causing the great loose of yield and implies the risk of their genetic vulnerability. Thus, the knowledge of genetic diversity of barly germplasm is important for breeding programmes and their conservation. Random amplified polymorphic DNA (RAPD) and SDS-PAGE techniques were used to examine the genetic variation among barley varieties. To maximize the genome coverage nine barley varieties from diverse origins were selected and analyzed with 18 selected RAPD markers. A total of 116 amplified bands and 63 polymorphic allelic variants were detected, ranging from 3 to 6 per locus with an average of 3.937. Genetic distance calculated from RAPD data ranged from 0.357 to 0.767. The dendrogram based on genetic distance estimation and PCA was produced two groupings that generally consistent with available genetic information. Zanbaka and Tadmor/roh6 were found to be closely related. Their genetic relationship was also confirmed by the facts that they shared a common ancestor and they were clustered together. A total of 14 unique bands (8 positives and 6 negatives) were identified out of 116 bands which could be considered for marker assisted selection. A slight range of variation in SDS-protein banding patterns was observed. A total number of 12 bands with different molecular weights were observed in SDS-PAGE, among these, only 5 bands were polymorphic. The dendrogram tree and PCA analysis showed that Elkheir and Canela were genetically distanced from the other genotypes. According to the results of RAPD and SDS-PAGE, a wide range of genetic diversity was observed among all varieties, indicating them as prime candidates of selective breeding for specific traits and broadening the genetic base. Penerbit Universiti Kebangsaan Malaysia 2014-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7295/1/43_1_12.pdf N.A., Tahir (2014) Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.). Malaysian Applied Biology, 43 (1). pp. 107-117. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Genetic variability of barley (Hordeum spp) considered very important, reducing their genetic diversity affects negatively their tolerance to stress factors, causing the great loose of yield and implies the risk of their genetic vulnerability. Thus, the knowledge of genetic diversity of barly germplasm is important for breeding programmes and their conservation. Random amplified polymorphic DNA (RAPD) and SDS-PAGE techniques were used to examine the genetic variation among barley varieties. To maximize the genome coverage nine barley varieties from diverse origins were selected and analyzed with 18 selected RAPD markers. A total of 116 amplified bands and 63 polymorphic allelic variants were detected, ranging from 3 to 6 per locus with an average of 3.937. Genetic distance calculated from RAPD data ranged from 0.357 to 0.767. The dendrogram based on genetic distance estimation and PCA was produced two groupings that generally consistent with available genetic information. Zanbaka and Tadmor/roh6 were found to be closely related. Their genetic relationship was also confirmed by the facts that they shared a common ancestor and they were clustered together. A total of 14 unique bands (8 positives and 6 negatives) were identified out of 116 bands which could be considered for marker assisted selection. A slight range of variation in SDS-protein banding patterns was observed. A total number of 12 bands with different molecular weights were observed in SDS-PAGE, among these, only 5 bands were polymorphic. The dendrogram tree and PCA analysis showed that Elkheir and Canela were genetically distanced from the other genotypes. According to the results of RAPD and SDS-PAGE, a wide range of genetic diversity was observed among all varieties, indicating them as prime candidates of selective breeding for specific traits and broadening the genetic base.
format	Article
author	N.A., Tahir
spellingShingle	N.A., Tahir Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
author_facet	N.A., Tahir
author_sort	N.A., Tahir
title	Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
title_short	Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
title_full	Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
title_fullStr	Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
title_full_unstemmed	Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)
title_sort	comparison of rapd-pcr and sds-page techniques to evaluate genetic variation among nine barley arieties (hordeum spp.)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/ http://journalarticle.ukm.my/7295/1/43_1_12.pdf
first_indexed	2023-09-18T19:49:18Z
last_indexed	2023-09-18T19:49:18Z
_version_	1777406111964463104

Comparison of RAPD-PCR and SDS-Page techniques to evaluate genetic variation among nine barley arieties (Hordeum spp.)

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