Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219

In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light...

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Main Authors:	Che Radziah, C.M.Z., Siti Nurkhalida, A.K., Zamri, Z., Ismanizan, I.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/7422/ http://journalarticle.ukm.my/7422/ http://journalarticle.ukm.my/7422/1/41_1_5.pdf

id	ukm-7422
recordtype	eprints
spelling	ukm-74222016-12-14T06:44:02Z http://journalarticle.ukm.my/7422/ Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219 Che Radziah, C.M.Z. Siti Nurkhalida, A.K. Zamri, Z. Ismanizan, I. In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline. Penerbit Universiti Kebangsaan Malaysia 2012-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7422/1/41_1_5.pdf Che Radziah, C.M.Z. and Siti Nurkhalida, A.K. and Zamri, Z. and Ismanizan, I. (2012) Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219. Malaysian Applied Biology, 41 (1). pp. 37-41. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL-1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.
format	Article
author	Che Radziah, C.M.Z. Siti Nurkhalida, A.K. Zamri, Z. Ismanizan, I.
spellingShingle	Che Radziah, C.M.Z. Siti Nurkhalida, A.K. Zamri, Z. Ismanizan, I. Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
author_facet	Che Radziah, C.M.Z. Siti Nurkhalida, A.K. Zamri, Z. Ismanizan, I.
author_sort	Che Radziah, C.M.Z.
title	Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
title_short	Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
title_full	Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
title_fullStr	Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
title_full_unstemmed	Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219
title_sort	effect of illumination, casein hydrolysate and proline on callus induction of oryza sativa l. var. mr219
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/7422/ http://journalarticle.ukm.my/7422/ http://journalarticle.ukm.my/7422/1/41_1_5.pdf
first_indexed	2023-09-18T19:49:38Z
last_indexed	2023-09-18T19:49:38Z
_version_	1777406133080686592

Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. var. MR219

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