Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering
The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular we...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2010
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| Online Access: | http://journalarticle.ukm.my/7441/ http://journalarticle.ukm.my/7441/ http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf |
| Summary: | The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 °— 106 and 1.87 ± 0.4 °— 105 gmol-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides. |
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