Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering
The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular we...
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Universiti Kebangsaan Malaysia
2010
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ukm-74412016-12-14T06:44:06Z http://journalarticle.ukm.my/7441/ Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering Zainon Othman, Saphwan Al-Assaf, Osman Hassan, The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 °— 106 and 1.87 ± 0.4 °— 105 gmol-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf Zainon Othman, and Saphwan Al-Assaf, and Osman Hassan, (2010) Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering. Sains Malaysiana, 39 (6). pp. 969-973. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html |
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description |
The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 °— 106 and 1.87 ± 0.4 °— 105 gmol-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides. |
format |
Article |
author |
Zainon Othman, Saphwan Al-Assaf, Osman Hassan, |
spellingShingle |
Zainon Othman, Saphwan Al-Assaf, Osman Hassan, Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
author_facet |
Zainon Othman, Saphwan Al-Assaf, Osman Hassan, |
author_sort |
Zainon Othman, |
title |
Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
title_short |
Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
title_full |
Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
title_fullStr |
Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
title_full_unstemmed |
Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
title_sort |
molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering |
publisher |
Universiti Kebangsaan Malaysia |
publishDate |
2010 |
url |
http://journalarticle.ukm.my/7441/ http://journalarticle.ukm.my/7441/ http://journalarticle.ukm.my/7441/1/26_Ayiesah.pdf |
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2023-09-18T19:49:44Z |
last_indexed |
2023-09-18T19:49:44Z |
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