Xylanase gene from a locally isolated bacterium

A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylan...

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Main Authors:	Hussain, M.H., Chong, N.F.M., Chan, C.S.W., Safarina, A., Husaini, A.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/7551/ http://journalarticle.ukm.my/7551/ http://journalarticle.ukm.my/7551/1/06_hasnain.pdf

id	ukm-7551
recordtype	eprints
spelling	ukm-75512016-12-14T06:44:26Z http://journalarticle.ukm.my/7551/ Xylanase gene from a locally isolated bacterium Hussain, M.H. Chong, N.F.M. Chan, C.S.W. Safarina, A. Husaini, A. A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 650 bp was successfully amplified using PCR and cloned into Escherichia coli XL-1 Blue. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1,4-beta-xylanase gene from the family 11 glycoside hydrolase. It showed 98% homology with Bacillus subtilis xylanase gene. In silico characterisation showed an open reading frame encoding a 213 amino acid sequence with a molecular weight of 23.3 kDa and theoretical isolectric point (pI) at pH 9.42. Penerbit Universiti Kebangsaan Malaysia 2011 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7551/1/06_hasnain.pdf Hussain, M.H. and Chong, N.F.M. and Chan, C.S.W. and Safarina, A. and Husaini, A. (2011) Xylanase gene from a locally isolated bacterium. Malaysian Applied Biology, 40 (1). pp. 33-38. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 650 bp was successfully amplified using PCR and cloned into Escherichia coli XL-1 Blue. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1,4-beta-xylanase gene from the family 11 glycoside hydrolase. It showed 98% homology with Bacillus subtilis xylanase gene. In silico characterisation showed an open reading frame encoding a 213 amino acid sequence with a molecular weight of 23.3 kDa and theoretical isolectric point (pI) at pH 9.42.
format	Article
author	Hussain, M.H. Chong, N.F.M. Chan, C.S.W. Safarina, A. Husaini, A.
spellingShingle	Hussain, M.H. Chong, N.F.M. Chan, C.S.W. Safarina, A. Husaini, A. Xylanase gene from a locally isolated bacterium
author_facet	Hussain, M.H. Chong, N.F.M. Chan, C.S.W. Safarina, A. Husaini, A.
author_sort	Hussain, M.H.
title	Xylanase gene from a locally isolated bacterium
title_short	Xylanase gene from a locally isolated bacterium
title_full	Xylanase gene from a locally isolated bacterium
title_fullStr	Xylanase gene from a locally isolated bacterium
title_full_unstemmed	Xylanase gene from a locally isolated bacterium
title_sort	xylanase gene from a locally isolated bacterium
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/7551/ http://journalarticle.ukm.my/7551/ http://journalarticle.ukm.my/7551/1/06_hasnain.pdf
first_indexed	2023-09-18T19:50:00Z
last_indexed	2023-09-18T19:50:00Z
_version_	1777406155973197824

Xylanase gene from a locally isolated bacterium

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